| a universal pcr primer to detect members of the potyviridae and its use to examine the taxonomic status of several members of the family. | a universal primer (sprimer: 5'-ggx aay aay agy ggx caz cc-3', x = a, g, c or t; y = t or c; z = a or g), designed from the consensus sequences that code for the conserved sequence gnnsgqp in the nib region of members of the family potyviridae, was used to amplify by rt-pcr the 3'-terminal genome regions from infected plant samples representing 21 different viruses in the family. sequencing of some of the fragments (c. 1.7 kb) showed that the type strain (attc pv-107) of oat necrotic mottle viru ... | 2001 | 11402861 |
| molecular characterization and evolutionary analysis of soybean mosaic virus infecting pinellia ternata in china. | twenty-nine pinellia ternata specimens were collected from representative areas in china, including the major production provinces of zhejiang, henan, shanxi, hunan, shandong and hubei. seven isolates related to soybean mosaic virus (smv), which could be pathogenic on p. ternata and some soybean [glycine max (l.) merr.] cultivars, were detected using double antibody sandwich immunosorbent assay (das-elisa) and rt-pcr amplification performed with degenerate primer of potyviruses. it is revealed t ... | 2008 | 18058013 |