| mitochondrial dna differentiation in the japanese brown frog rana japonica as revealed by restriction endonuclease analysis. | to elucidate mtdna differentiation in the japanese brown frog rana japonica, and compare it with results from allozyme analysis and crossing experiments, rflp analysis was conducted on 78 frogs from 16 populations in honshu. purified mtdna was digested with eight six-base recognizing restriction enzymes and analyzed by 1% agarose-slab gel electrophoresis. cleavage patterns of the mtdna showed three distinct genome size classes: small (18.5 kb), middle (20.0 kb) and large (21.5 kb). ten haplotype ... | 1997 | 9265735 |