Publications

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toxicity of cyanide, iron-cyanide complexes, and a blast-furnace effluent to the banana prawn, penaeus monodon. 19979115149
molecular characterisation of hepatopancreatic parvovirus (pmergdnv) from australian penaeus merguiensis.hepatopancreatic parvovirus infection is associated with reduced growth rates of prawns during the juvenile stages and overt mortalities. hepatopancreatic parvovirus was purified from penaeus merguiensis from northern queensland and a partial consensus sequence of 5.9 kb was obtained. nucleotide comparisons revealed that the australian isolate of hpv has a nucleotide similarity (87%) closer to hpvchin and the full sequence of hpv penaeus monodon (pmdnv) (6321 bp) than to hpvsemi (83%). three put ...200717275056
in situ stress testing to identify australian black tiger prawns (penaeus monodon) free of gill-associated virus and mourilyan virus.to identify whether black tiger prawns (penaeus monodon) in the weipa region of the gulf of carpentaria, queensland, are free of gill-associated virus (gav) and mourilyan virus (mov), which are endemic in p. monodon along the east coast of queensland.200919489783
vibrio owensii sp. nov., isolated from cultured crustaceans in australia.two bacterial strains (dy05(t) and 47666-1) were isolated in queensland, australia, from diseased cultured crustaceans panulirus ornatus and penaeus monodon, respectively. on the basis of 16s rrna gene sequence identity, the strains were shown to belong to the harveyi clade of the genus vibrio. multilocus sequence analysis using five housekeeping genes (rpoa, pyrh, topa, ftsz and mreb) showed that the strains form a monophyletic group with 94.4% concatenated sequence identity to the closest spec ...201020002183
vertical transmission of gill-associated virus (gav) in the black tiger prawn penaeus monodon.chronic gill-associated virus (gav) infection is endemic in penaeus monodon broodstock captured from north-east queensland in australia and in farmed shrimp produced from these. we investigated the role of vertical transmission in perpetuating the high prevalence of these chronic gav infections. reverse transcription (rt)-nested pcr detected gav in spermatophores and mature ovarian tissue from broodstock and in fertilized eggs and nauplii spawned from wild-fertilized females. in laboratory-reare ...200212180710
detection of australian gill-associated virus (gav) and lymphoid organ virus (lov) of penaeus monodon by rt-nested pcr.a highly sensitive test based on reverse transcription followed by nested polymerase chain reaction (rt-npcr) was developed to detect the australian yellow-head-like viruses, gill-associated virus (gav) and lymphoid organ virus (lov) of penaeus monodon. the rt-npcr detected viral rna in as little as 10 fg lymphoid organ total rna isolated from gav-infected p. monodon. amplification of serial dilutions of a gav cdna clone showed that the nested pcr was sufficiently sensitive to detect a single ge ...200010768283
new yellow head virus genotype (yhv7) in giant tiger shrimp penaeus monodon indigenous to northern australia.in 2012, giant tiger shrimp penaeus monodon originally sourced from joseph bonaparte gulf in northern australia were examined in an attempt to identify the cause of elevated mortalities among broodstock at a queensland hatchery. nucleic acid extracted from ethanol-fixed gills of 3 individual shrimp tested positive using the oie yhv protocol 2 rt-pcr designed to differentiate yellow head virus (yhv1) from gill-associated virus (gav, synonymous with yhv2) and the oie yhv protocol 3 rt-nested pcr d ...201526290511
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