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high prevalence of toxoplasma gondii infection in microtus fortis by seminested pcr from jilin province, northeastern china.toxoplasma gondii is an important protozoan parasite that can infect virtually all warm-blooded animals, including humans. oocysts in the environment are an important infection source. microtus fortis can be used as a sentinel reflecting the distribution of t. gondii oocysts in the environment indirectly. in the present study, we developed a seminested polymerase chain reaction (pcr) targeting t. gondii 529-base-pair repeat sequence, with high sensitivity and specificity. two hundred seventy-two ...201323050695
first report of genotyping of toxoplasma gondii in free-living microtus fortis in northeastern china.toxoplasma gondii is an intracellular protozoan parasite which imperils the health of almost all warm-blooded animals, including humans. the objective of this study was to determine genetic characterization of t. gondii in free-living microtus fortis (reed vole) in jilin province, northeastern china. a total of 104 dna samples, 74 from gongzhuling and 30 from baicheng, were extracted from lung tissues of m. fortis , and 56 (53.8%) of them were positive for t. gondii by semi-nested polymerase cha ...201424684624
isolation and genetic characterization of hantaviruses carried by microtus voles in china.to gain more insights into hantavirus distribution in china, microtus fortis were caught in jilin province and m. maximowiczii in the inner mongolia autonomous region. hantavirus specific rna was detected by rt-pcr in 3 out of 26 m. fortis and 5 out of 64 m. maximowiczii. two hantaviruses (fusong-mf-682 and yakeshi-mm-59) were isolated successfully in cell culture and their s and m segment nucleotide sequences were determined. phylogenetic analysis of the s and m segment sequences revealed that ...200818297708
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