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molecular phylogenetic analysis of theileria species detected from japanese serows (capricornis crispus) in iwate prefecture, japan.in the present study, we tried to detect dna for ribosomal rna genes of piroplasma parasites from the liver or blood of 43 japanese serows (capricornis crispus) in iwate prefecture of japan by polymerase chain reaction. approximately 500-bp amplicons were obtained in 35 (81.4%) of the 43 samples by amplification for v4 hyper-variable regions of the 18s rrna gene, and the amplicons were considered to be dna of theileria species. the complete nucleotide sequences (1,700 or 1,709 bp) of the 18s rrn ...201121646752
distinct distribution of dicrocoelium dendriticum and d. chinensis in iwate prefecture, japan, and a new final host record for d. chinensis.this study dealt with the morphological and molecular identification of dicrocoelium flukes obtained from japanese serow (capricornis crispus) and sika deer (cervus nippon centralis) in the twelve districts of iwate prefecture, japan. dicrocoelium dendriticum and d. chinensis were exclusively detected in the western, and coastal and eastern areas of iwate prefecture, respectively. this geographically distinct occurrence of the two dicrocoelium species would be associated with the distribution of ...201424998394
a new oxyurid species (nematoda) collected from a japanese serow, capricornis crispus (mammalia: bovidae), in japan.skrjabinema kamosika sp. n. (nematoda: oxyuridae) is described based on both sexes collected from endemic japanese serows, capricornis crispus (mammalia: bovidae), of wakayama prefecture, western honshu island, and kochi prefecture, shikoku island, japan. it is readily distinguished from the congeners by having tricuspid sub-interlabial projections in males, large triangular lateral lobes of the lips that do not reach the mouth rim, and a large length ratio of alate/nonalate portions of the tail ...201222662765
mycoplasma ovis detected in free-living japanese serows, capricornis crispus.nineteen blood samples collected from free-ranging wild japanese serows, capricornis crispus, between 2006 and 2008 in iwate prefecture were examined for the hemoplasma infection by real-time pcr targeting the 16s rrna gene. five (26.3%) out of the 19 samples were positive in real-time pcr with an average melting temperature at 85.18 °c. the positive samples in the real-time pcr were reconfirmed by conventional pcr, and one of them was successful for direct dna sequencing. the nucleotide sequenc ...201020962460
phylogenetic analysis of the 16s rrna gene of anaplasma species detected from japanese serows (capricornis crispus).nineteen blood samples collected from free-living japanese serows, capricornis crispus, between 2006 and 2008 in iwate prefecture were examined for the anaplasma infection by pcr amplification of a part of the 16s rrna gene. ten (52.6%) out of the 19 samples produced a visible band in electrophoresed agarose gels. positive pcr products were subjected to dna sequencing. we found the nucleotide sequences were identical. almost entire length of the 16s rrna gene for a representative stain was then ...200920046041
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