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improvement and optimization of a multiplex real-time reverse transcription polymerase chain reaction assay for the detection and typing of vesicular stomatitis virus.an improvement to a previously reported real-time reverse transcription polymerase chain reaction (real-time rt-pcr) assay for the detection of vesicular stomatitis virus (vsv) is described. results indicate that the new assay is capable of detecting a panel of genetically representative strains of vsv present in north, central, and south america. the assay is specific for vsv and allows for simultaneous differentiation between vesicular stomatitis indiana virus and vesicular stomatitis new jers ...201020453220
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