| development of polymerase chain reaction for specific identification of epizootic hemorrhagic disease virus serotype 1. | the diagnostic potential of the polymerase chain reaction (pcr) for specific identification of epizootic hemorrhagic disease virus serotype 1 (ehdv-1) in cell culture and clinical specimens was evaluated. using oligonucleotide primers, selected from genome segment 2 of ehdv-1 (new jersey strain), the pcr-based assay resulted in a 862 base pair (bp) pcr product. ehdv-1 rna from united states prototype serotype 1 and a number of ehdv-1 field isolates, propagated in cell cultures, were detected by ... | 1995 | 8572948 |