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heavy metal concentrations in marine fishes collected from fish culture sites in hong kong.the levels of six heavy metals (cd, cr, cu, ni, pb, and zn) in different tissues of three species of cultured marine fishes (epinephelus areolatus, lutjanus russelli, and sparus sarba) collected from three fish culture sites in hong kong were evaluated. metal pollution problems in the fish culture sites were serious, as reflected by the high metal concentrations recorded in sea water, sediments, and the biomonitor perna viridis. in general, tissues of all three species contained high concentrati ...200111116341
an overview of trace metal pollution in the coastal waters of hong kong.the state of metal pollution in hong kong's coastal waters has been assessed by measuring metal levels in: (i) the water column; (ii) sediments and (iii) in organisms, i.e. biomonitors. current literature is reviewed. data from sediment analysis have shown that metal pollution is most severe in the urban areas of victoria harbour, tolo harbour, deep bay and northwestern waters. bottom sediments in typhoon shelters are particularly heavily polluted with, for example, cu levels from kowloon bay re ...19989646516
concentrations of polycyclic aromatic hydrocarbons and polychlorinated biphenyls in green-lipped mussel perna viridis from victoria harbour, hong kong and possible human health risk. 200919155024
influences of ammonia-nitrogen and dissolved oxygen on lysosomal integrity in green-lipped mussel perna viridis: laboratory evaluation and field validation in victoria harbour, hong kong.lysosomal integrity in mussels has been applied as a biomarker to detect the pollution of trace organics and metals in the natural environments. however, few studies have examined the effects of water quality on the response of lysosomal integrity, in particular total ammonia-nitrogen (tan) and dissolved oxygen (do). this study demonstrated that high level of tan (2.0mg/l) and low do (2.5mg o(2)/l) could significantly reduce the lysosomal integrity in green-lipped mussel perna viridis, respectiv ...200818789457
metal concentrations in green-lipped mussels (perna viridis) and rabbitfish (siganus oramin) from victoria harbour, hong kong after pollution abatement. 200818554668
the use of selected genotoxicity assays in green-lipped mussels (perna viridis): a validation study in hong kong coastal waters.in hong kong, discharges of domestic and industrial wastewaters and disposal of contaminated muds into coastal waters have resulted in high levels of persistent organic contaminants in the water column, biota and bottom sediments. although the acute toxicity of effluents has been tested by government regulators using mortality-based bioassays, the sublethal effects of many of the pollutants, including environmental carcinogens, have not been well studied. the objective of the present work was to ...200818420224
polycyclic musks in green-lipped mussels (perna viridis) from hong kong.six polycyclic musk compounds [cashmeran (dpmi), celestolide (adbi), phantolide (ahmi), traseolide (atii), tonalide (ahtn), and galaxolide (hhcb)] were analysed in marine green-lipped mussels (perna viridis) from hong kong. adbi, hhcb and ahtn were detected in almost all samples, while ahmi, atii and dpmi were not detected. concentrations of adbi, hhcb and ahtn in mussels ranged from below detection limit-0.0743 (mean: 0.0246), 0.247-6.08 (mean: 1.15) and 0.0591-0.738 (mean: 0.190)mg/kg lipid we ...200818384818
field observations on correlation of fatty acid profiles between suspended particulate matter and green-lipped mussels in subtropical waters of hong kong.by analysis of the fatty acid profiles in mussel tissues and suspended particulate matter (spm) in water, the present study showed a significant relationship of the trophic linkage between mussels and the spm. at seven locations from inner to outer areas along the eutrophic tolo harbour and tolo channel, hong kong, the composition (as percentage of total fatty acids) of both monoenoic and polyunsaturated fatty acids in the tissues of green-lipped mussels perna viridis and spm in water had signif ...200818289610
biokinetics of paralytic shellfish toxins in the green-lipped mussel, perna viridis. 200717391712
field validation of antioxidant enzyme biomarkers in mussels (perna viridis) and clams (ruditapes philippinarum) transplanted in hong kong coastal waters.green-lipped mussels, perna viridis, and manila clams, ruditapes philippinarum were sourced from "clean" sites in the hong kong region, depurated in a laboratory using uncontaminated filtered seawater for 8 days, and transplanted to a suspected gradient of chemically polluted sites in hong kong. after 14- and 28-days of field exposure, several antioxidant parameters including glutathione s transferase (gst), catalase (cat), glutathione peroxidase (gpx), and glutathione (gsh) were quantified in g ...200516291185
okadaic acid, a causative toxin of diarrhetic shellfish poisoning, in green-lipped mussels perna viridis from hong kong fish culture zones: method development and monitoring.green-lipped mussels (perna viridis) were collected from seven fish culture zones (fczs) in hong kong and analyzed for okadaic acid (oa). a conventional hplc method was modified by incorporating a proteinase k digestion step. results suggest that a higher recovery (2.5 times higher) of oa was obtained from spiked samples after the addition of 1.08 mg proteinase k in comparison with samples incubated without the proteolytic enzyme. for the hepatopancreas (hp) of individual field-collected mussels ...200516040057
polybrominated diphenyl ethers (pbdes) in sediments and mussel tissues from hong kong marine waters.sediments and green-lipped mussels, perna viridis, were used to investigate concentrations of polybrominated diphenyl ethers (pbdes) in hong kong's marine environment. pbdes have been used extensively over the past two decades as flame retardants in polymer additives for a variety of plastics, computers, furniture, building materials, and fabrics. many measurements of pbdes in various environmental matrices have been reported from belgium, holland, japan, europe and north america, but few measur ...200515950246
micronucleus induction in gill cells of green-lipped mussels (perna viridis) exposed to mixtures of polycyclic aromatic hydrocarbons and chlorinated pesticides.different groups of green-lipped mussels (perna viridis) were exposed to the same net amount of a genotoxicant mixture of four polycyclic aromatic hydrocarbons ([pahs]; anthracene, fluoranthene, pyrene, and benzo[a]pyrene) and four organochlorine pesticides ([ocs]; alpha-hexachlorocyclohexane (hch), aldrin, dieldrin, and p,p'-ddt) for four weeks under different regimes that simulated various scenarios of fluctuating toxicant levels in the marine environment. micronucleus (mn) formation in gill c ...200415180386
application of the comet and micronucleus assays to the detection of b[a]p genotoxicity in haemocytes of the green-lipped mussel (perna viridis).green-lipped mussels (perna viridis) were exposed to water-borne benzo[a]pyrene (b[a]p) at nominal concentrations of 0, 0.3, 3 and 30 microg l(-1) for up to 12 days, and both the relative levels of dna strand breaks (assessed using an alkaline comet assay) and the proportion of micronucleus (mn) formation were monitored in mussel haemocytes at days 0, 1, 3, 6 and 12. the results of the comet assay indicated that an increase in the proportion of strand breaks occurred generally with increasing b[ ...200415168946
influences of different selenium species on the uptake and assimilation of hg(ii) and methylmercury by diatoms and green mussels.we examined the influences of different concentrations and species of se (selenite, selenate, and seleno-l-methionine) in the ambient environment on the accumulation of inorganic hg(ii) and methylmercury (mehg) by the diatom thalassiosira pseudonana and the green mussel perna viridis. at the experimental concentrations tested (< 500 microgl(-1)), selenite and selenate did not significantly affect the uptake of either mercury species by the diatoms and the green mussels. the assimilation efficien ...200415110468
accumulation of metals in the soft tissues, byssus and shell of the mytilid mussel perna viridis (bivalvia: mytilidae) from polluted and uncontaminated locations in hong kong coastal waters. 200312907199
lysosomal membrane stability, phagocytosis and tolerance to emersion in the mussel perna viridis (bivalvia: mytilidae) following exposure to acute, sublethal, copper.the mytilid mussel perna viridis is distributed throughout the indo-pacific region and is potentially a suitable candidate for biological effects (biomarker) monitoring in the subtropics. a suite of cytological and physiological responses to acute (48-72 h) copper exposures of 50-200 microgl(-1) were assessed in order to determine the suitability of p. viridis for marine pollution monitoring. copper elicited significant destabilisation of the haemocyte lysosomal membranes and also impaired phago ...200312820995
paralytic shellfish toxins in green-lipped mussels, perna viridis, in hong kong. 200312586122
comparison of metal accumulation in mussels at different local and global scales.cadmium and zinc uptake from the dissolved phase, assimilation efficiency (ae) from the dietary phase, and body burden as well as clearance rate were measured in green mussels, perna viridis, and blue mussels mytilus edulis, m. galloprovincialis and mytilus trossulus. perna viridis was collected from four sites differentially enriched with trace metals in hong kong and blue mussels were collected from different climatic zones, i.e., subarctic and temperate, to allow comparisons with the more tro ...200312558172
a comparison of mussels (perna viridis) and semi-permeable membrane devices (spmds) for monitoring chlorinated trace organic contaminants in hong kong coastal waters.a comparison of mussels (perna viridis) and semi-permeable membrane devices (spmds) was carried out at five sites, representing a gradient of contaminant concentrations, in hong kong coastal waters. mussels, originally collected from a "clean" location, were deployed along with spmds at each site for 30 days. analyses for chlorinated pesticides and polychlorinated biphenyls (pcbs) indicated that spmds have potential as monitoring tools, and to some extent can overcome the problems associated wit ...200111695633
relationships between tissue concentrations of polycyclic aromatic hydrocarbons and antioxidative responses of marine mussels, perna viridis.local mussels, perna viridis, were transplanted from a relatively clean site to various polluted sites in hong kong. after a 30-day field exposure, different antioxidant parameters including glutathione s transferase (gst), superoxide dismutase (sod), catalase (cat), glutathione peroxidase (gpx), glutathione reductase (gr), nadph dt-diaphorase (dt-d), glutathione (gsh) and lipid peroxidation were quantified, and tissue concentrations of benzo[a]pyrene (b[a]p) as well as a total of five polycycli ...200111239681
power analysis for biomarkers in mussels for use in coastal pollution monitoring.data from literature on neutral red retention time (nrrt) in lysosomes, micronucleus (mn) frequency and condition index (ci) in mussel mytilus, especially mytilus edulis and mytilus galloprovincialis, were re-analyzed to ascertain their statistical power in detecting a minimum 20% spatial/temporal change in field studies. results showed that ci largely displayed higher statistical power (>90%) than lysosomal nrrt and mn frequency (<50%), suggesting that data from the latter two biomarkers may le ...200919406439
organochlorines and dioxin-like compounds in green-lipped mussels perna viridis from hong kong mariculture zones.concentrations of persistent organic pollutants including polychlorinated biphenyls (pcbs), organochlorine (oc) pesticides and dioxin-like compounds were measured in green-lipped mussels, perna viridis, collected from seven mariculture zones in hong kong between september and october in 2002 in order to evaluate the status, spatial distribution and potential sources of pollution in these areas. concentrations ranged from 300 to 4400 ng/g lipid weight for total ocs and 170-1000 ng/g lipid weight ...200516023147
biomonitoring of heavy metals and trace organics using the intertidal mussel perna viridis in hong kong coastal waters.this paper presents the results of a 6-year (1998-2003) survey of trace toxics in the intertidal mussel perna viridis conducted by the hong kong environmental protection department. concentrations of heavy metals and trace organics were measured in the soft bodies of p. viridis collected from five sites in hong kong waters, i.e. wu kai sha (tolo harbour), ma wan (northwest), tsim sha tsui (victoria harbour), tai tam (hong kong south) and lamma island (southern waters) in order to establish the s ...200515907944
effect of size, tissue parts and location on six biochemical markers in the green-lipped mussel, perna viridis.the effect of size and tissue parts as well as site locations on a suite of six anti-oxidative biochemical markers, viz. the enzyme degrading neural transmitters acetylcholinesterase (ache), the phase ii transforming enzyme glutathione-s-transferase (gst), the anti-oxidative enzymes superoxide dismutase (sod) and catalase (cat), the lipid metabolite malondialdehyde (mda) and the ratio of cat/mda, in the bivalve mollusc, perna viridis, was studied. field mussels were collected from the piers of u ...200314643783
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