| amplification by long rt-pcr of near full-length norovirus genomes. | a long rt-pcr method was developed to amplify the norovirus genome. starting from rna extracted directly from clinical samples and using broadly reactive primers, it can generate near full-length amplicons that allow for easy determination of the near complete genomic sequence. two norovirus isolates from toronto, canada, in 2002 and 2005 were sequenced. this approach will facilitate molecular epidemiology studies of noroviruses. | 2008 | 18355931 |
| an evaluation of methods for the isolation of yersinia enterocolitica from surface waters in the grand river watershed. | yersinia enterocolitica is a foodborne pathogen, but the importance of water as a route of exposure for human infection is not well known. y. enterocolitica isolation methods were developed primarily for food and clinical samples, and may not be effective for use with environmental samples. the objective of this study was to assess the recovery of y. enterocolitica from surface water used for drinking water treatment. four enrichment broths and an alkaline treatment protocol were compared for th ... | 2009 | 19491491 |