| diagnosis of brazilian vesiculoviruses by reverse transcription-polymerase chain reaction. | we describe a reverse transcription-polymerase chain reaction (rt-pcr) and a nested-pcr for diagnosis of piry, carajás, cocal, and alagoas vesiculoviruses from brazil. the rna extracts of viral and clinical samples were submitted to a rt-pcr using vesiculovirus g primers that amplify part of the glycoprotein gene. the rt-pcr produced amplicons of expected size, 290 base pair, for the four studied viruses. the rt-pcr showed a high sensitivity being 151.3 times (2.18 log) more sensitive for the de ... | 2005 | 16021308 |