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direct 16s rrna gene sequencing from clinical specimens, with special focus on polybacterial samples and interpretation of mixed dna chromatograms.ripseq (isentio, bergen, norway) is a web-based application for the analysis of mixed dna chromatograms. it opens the possibility to analyze chromatograms obtained by direct 16s rrna gene sequencing from polybacterial human clinical samples. in this study, we used direct 16s rrna gene sequencing to investigate 264 samples from a wide range of suspected human bacterial infections. the sequence-based identification was compared with the results from routine culture-based identification. a total of ...200919741089
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