Publications

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occurrence of plasmids and tetracycline resistance among campylobacter jejuni and campylobacter coli isolated from whole market chickens and clinical samples.twenty whole market chickens, purchased from 10 different stores in the taipei metropolitan area, were examined for the presence of campylobacter jejuni and campylobacter coli. the microorganisms were recovered from 95% of the chickens. a survey of different sites on--breast, thigh and tail--showed that contamination was equally common on all these sites. one hundred and sixty-seven chicken isolates and the 41 clinical isolates of campylobacter jejuni were examined for the occurrence of plasmid ...19947703010
nationwide surveillance of antimicrobial resistance among enterobacteriaceae in intensive care units in taiwan.to determine the antimicrobial resistance profiles among clinical isolates of enterobacteriaceae in taiwanese intensive care units (icus), a national surveillance of antibiotic resistance among important enterobacteriaceae was conducted from september 2005 through november 2005 at the icus of ten major teaching hospitals in taiwan. a total of 574 enterobacteriaceae isolates recovered from various clinical samples of our icu patients were submitted for in vitro test. minimum inhibitory concentrat ...200918716805
decreased grp78 protein expression is a potential prognostic marker of oral squamous cell carcinoma in taiwan.oral squamous cell carcinoma (oscc) is an aggressive tumor and its occurrence in taiwan is closely related to chronic smoking, alcohol consumption, and especially to betel quid chewing. it became the fourth most common malignant tumor of taiwanese men in 2006. unfortunately, there are few biomarkers for diagnosis and treatment of this disease.201020497865
molecular detection and prevalence of enterovirus within environmental water in taiwan.to investigate the presence of enterovirus rna in various samples of environmental water collected in taiwan during 2004-2005, and to characterize the genotypes and distribution of the viruses identified in taiwan.200818201176
genetic diversity of epidemic enterovirus 71 strains recovered from clinical and environmental samples in taiwan.most enteroviruses excreted in human feces and urine are present in environmental water. in order to clarify the infection route of enterovirus, the detection of viruses in both clinical and environmental samples may contribute to understanding the mode of transmission of strains responsible for human infection. thus, 21 epidemic enterovirus 71 strains from environmental water or stool samples were collected from hfmd children during 2005. enterovirus genomic rna was first amplified directly fro ...200717363101
quantitation of severe acute respiratory syndrome coronavirus genome by real-time polymerase chain reaction assay using minor groove binder dna probe technology.the ability to rapidly recognize severe acute respiratory syndrome coronavirus (sars-cov) as a cause of infections is critical to quickly limiting further spread of the disease. a rapid, sensitive, and specific laboratory diagnostic test is needed to confirm outbreaks of sars-cov infection and distinguish it from other diseases that can cause similar clinical symptoms. an improved taqman technology using minor groove binder (mgb) probes was used to detect and quantify sars-cov in suspected patie ...200415497005
comparison of type a enterotoxigenic staphylococcus aureus strains isolated from geographically far distant locations by pulsed field gel electrophoresis.staphylococcal enterotoxin a (sea) is one of the major staphylococcal enterotoxins which may cause food-borne outbreaks. in order to investigate the difference in genomic types and to elucidate the most disseminated strains for enterotoxin a-producing strains of staphylococcus aureus, a total of 60 sea staph. aureus strains isolated from food and clinical samples in taiwan and 30 strains of the same enterotoxigenic type of strains obtained from geographically far distant locations were compared ...19979134722
high levels of meca dna detected by a quantitative real-time pcr assay are associated with mortality in patients with methicillin-resistant staphylococcus aureus bacteremia.persistent methicillin-resistant staphylococcus aureus (mrsa) bacteremia is known to be a poor prognostic factor. while several pcr assays for the detection of mrsa in various clinical samples were recently reported, the possibility that a quantitative pcr assay could be used to quantify and monitor mrsa bacteremia has not been explored. in this study, we established a quantitative real-time pcr assay for the meca gene using known copy numbers of a plasmid containing meca dna as a standard and t ...200919279177
safrole-dna adduct in hepatocellular carcinoma associated with betel quid chewing.betel quid chewing, which contributes high concentration of safrole in saliva, is a popular oral habit in taiwan. safrole is a documented rodent hepatocarcinogen, yet its hepatocarcinogenic potential in human is not known. here, we used lc/esi-itms(n) and lc/qtof-ms confirmed safrole-dgmp as reference standard to detect the safrole-dna adduct in hepatic tissues from hbsag-/hcv-seronegative hepatocellular carcinoma patients by (32)p-postlabeling. we first synthesized and confirmed safrole-dgmp by ...200818938230
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