| detection of salmonella inva gene in shrimp enrichment culture by polymerase chain reaction. | contamination of seafood with salmonellae is a major public health concern. detection of salmonella by standard culture methods is time consuming. in this study, an enrichment culture step prior to polymerase chain reaction (pcr) was applied to detect 284 bp fragment of salmonella inva in comparison with the conventional culture method in 100 shrimp samples collected from four different shrimp farms and fresh food markets around bangkok. samples were pre-enriched in non-selective lactose broth ( ... | 2010 | 20578527 |
| typing of chlamydia trachomatis strains from urine samples by amplification and sequencing the major outer membrane protein gene (omp1). | to develop a novel protocol for the extraction, amplification, and sequencing of chlamydia trachomatis momp gene (omp1) from urine, a non-invasive source, and apply it to an epidemiological study on the distribution of c trachomatis strains in a population of pregnant women in thailand. | 2001 | 11714939 |
| a new quantitative rt-pcr method for sensitive detection of dengue virus in serum samples. | in order to detect and identify dengue serotypes in serum samples, we developed a single-step quantitative reverse transcription-polymerase chain reaction (q-rt-pcr) assay (referred to as q-pcr). sets of primers were selected from the capsid region of the viral genome. dengue serotypes 1/3 and 2/4 were detected in two separate duplex amplification reactions using specific primers and fluorogenic taqman probes. results obtained with this q-pcr and the classical nested rt-pcr (n-pcr) assays were c ... | 2008 | 18652847 |