| rapid detection and characterization of chikungunya virus by rt-pcr in febrile patients from kerala, india. | there has been a resurgence and prevalence of fever with symptoms of chikungunya (chik) and increased death toll in kerala, the southern-most state of india. the objective of this study was to develop a rapid detection method to determine the presence of chik- virus in the serum samples collected from febrile patients in kerala, india. serum specimens were analyzed for chik viral rna by rt-pcr using primers specific for nsp1 and e1 genes. five out of twenty clinical samples were positive for chi ... | 2008 | 18814485 |
| multiplex pcr on leptospiral isolates from kolenchery, kerala, india. | human leptospirosis causes severe multiorgan dysfunctions that may end in multiorgan failure and death. the methods in hand for diagnosis of leptospirosis like culture, elisa and mat only help to confirm the disease, and are of little value in early detection. the aim of this study was to find out if the two sets of primers described earlier could detect all the isolates from the area, for the purpose of using the resultant database for early detection of leptospires in future from clinical spec ... | 2005 | 15928441 |