| prevalence of lyme borreliosis spirochetes in ixodid ticks of japan, with special reference to a new potential vector, ixodes ovatus (acari: ixodidae). | to determine the vectors of lyme borreliosis in japan, the ixodid ticks taken in hokkaido, fukushima, nagano, ehime, and kochi prefectures were examined individually for spirochetal infections by culturing their midgut tissues in bsk medium. the cultivable spirochetes identified as borrelia burgdorferi were detected in ixodes persulcatus schulze adults (15.5% of 634), nymph (6.7% of 15) and ixodes ovatus neumann adult (9.6% of 188) from hokkaido, i. ovatus adults (25.5% of 47) from fukushima, an ... | 1992 | 1495032 |
| antigenic properties of borrelia burgdorferi isolated from ixodes ovatus and ixodes persulcatus in hokkaido, japan. | spirochete strains hp3 and ho14, isolated from ixodes persulcatus and i. ovatus in hokkaido in 1989, were the first isolates of borrelia burgdorferi, the etiological agent of lyme disease, to be recognized in japan. antigenic properties of the japanese strains were compared with those of the strains isolated in the united states (b31 and 297) and europe (irs, p/gau, p/bi, 2/b45, and 3/b56) by western blotting (immunoblotting), by using monoclonal antibodies (mabs) against strains b31 and p/bi. t ... | 1991 | 1761676 |
| antibodies to borrelia burgdorferi in dogs in hokkaido. | during 1985 to 1990, serum samples were obtained from 229 healthy dogs. the dogs lived in hokkaido, known to be infested with ticks. an enzyme-linked immunosorbent assay (elisa) was used to detect igg and igm antibodies against borrelia burgdorferi ho14 and hp3, which were isolated from ixodes ovatus and i. persulcatus in the area. igg antibody to b. burgdorferi ho14 was detected in 8.8% (1985), 16.4% (1987) and 18.5% (1990). igm antibody to the bacteria was detected in 1.8% (1987) and 2.5% (199 ... | 1990 | 2098630 |
| infectivity and early antibody response to borrelia burgdorferi sensu lato isolated in japan in outbred mice. | borrelia burgdorferi sensu lato isolated from ixodes ovatus (b. japonica), i. persulcatus and patients with erythema migrans (em) in japan were determined on infectivity and arthritis induction-activity in outbred mice. infectivity of b. japonica was weak and did not induce the development of footpad swelling by subcutaneous (s.c.) inoculation into the footpad. challenged strain, no129-m of b. japonica, to ddy mice were reinoculated to the mice at various cell numbers (1 x 10-1 x 10(6) cells/mou ... | 1994 | 7799835 |
| isolation of tick-borne encephalitis virus from ixodes ovatus (acari: ixodidae) in japan. | a case of tick-borne encephalitis (tbe) was found in a farming area located in the southern part of hokkaido, japan, in 1993. tbe viruses were isolated from sentinel dogs in the area where the human case occurred in 1995. ticks were collected in the area by 2 collection methods in 1995 and virus isolation was conducted on ticks in 1995 and 1996. ixodes ovatus neumann was found to be the predominant tick species in the area. in 1996, 2 virus strains were isolated from 600 i. ovatus ticks (300 fem ... | 1998 | 9615539 |
| detection of dna of 'candidatus mycoplasma haemominutum' and spiroplasma sp. in unfed ticks collected from vegetation in japan. | dna fragments of 'candidatus mycoplasma haemominutum', a feline heamobartonella pathogen, were detected from unfed ixodes ovatus collected from vegetation in hokkaido, fukushima and yamaguchi prefectures, and unfed haemaphysalis flava in yamaguchi prefecture. this finding suggests that ixodid tick is a possible vector of 'c. mycoplasma haemominutum'. spiroplasma dna was also detected from unfed i. ovatus in hokkaido, fukushima and yamaguchi prefectures. the analysis of nucleotides sequence sugge ... | 2005 | 16397391 |
| prevalence of tick-borne rickettsia and ehrlichia in ixodes persulcatus and ixodes ovatus in tokachi district, eastern hokkaido, japan. | dna from 111 ticks collected by flagging in tokachi district, eastern hokkaido, japan were examined for infection with rickettsia and ehrlichia, by pcr and sequencing methodology. for rickettsia, analysis of the partial sequence of the citrate synthase gene was successfully performed on 11 dna samples from i. persulcatus, and 7 of them showed 99.8% identical with rickettsia helvetica while the other 4 showed 99.8% identical with ;candidatus rickettsia tarasevichiae'. for ehrlichia, a partial seq ... | 2007 | 17611367 |
| detection of anaplasma bovis and anaplasma phagocytophilum dna from haemaphysalis megaspinosa in hokkaido, japan. | dna from 101 ticks, including haemaphysalis megaspinosa, haemaphysalis douglasi, haemaphysalis flava, ixodes ovatus and ixodes persulcatus, collected by flagging in a pasture in hidaka district (hokkaido, japan) was examined for infection with anaplasma bovis and a. phagocytophilum by species-specific nested pcr. of the 48 h. megaspinosa nymphs, examined, 1 (2.1%) and 6 (12.5%) were positive for a. bovis and a. phagocytophilum, respectively. one of the 6 positive nymphs was dual positive for bot ... | 2010 | 19897306 |