| detection of ehrlichial infection by pcr in dogs from yamaguchi and okinawa prefectures, japan. | species-specific nested polymerase chain reaction (pcr) was used to detect the presence of possible canine ehrlichial agents (ehrlichia canis, e. chaffeensis, e. ewingii, e. equi and e. platys) and monocytic ehrlichial agents found in japan (e. muris and a recently discovered ehrlichia species detected from ixodes ovatus) in blood samples from dogs in yamaguchi and okinawa prefecture, japan. partial sequence of e. platys was detected from 1 of 67 dogs (1.5%) tested from yamaguchi prefecture and ... | 2001 | 11503912 |
| molecular survey of ehrlichia infection in ticks from animals in yamaguchi prefecture, japan. | a total of 82 ticks collected from wild animals and dogs in yamaguchi prefecture, japan were examined for ehrlichia infection by using polymerase chain reaction (pcr) primers that amplify dna of most members of the genus ehrlichia. a dna sample from an ixodes ovatus nymph from a bear in yamaguchi prefecture, japan, was positive in the screening pcr. subsequent pcr using two sets of primers yielded a 1431 bp segment of the 16s rrna gene and the sequence was very similar to those of e. chaffeensis ... | 2001 | 11511421 |
| tick infestation of sika deer (cervus nippon) in the western part of yamaguchi prefecture, japan. | ticks were collected from 94 sika deer (cervus nippon) hunted in the western part of yamaguchi prefecture, japan from august to november 1999, and march to july 2000. haemaphysalis longicornis and h. yeni were the dominant species from april to august, while h. flava and h. megaspinosa were dominant in october, november and march. this is the first report of h. yeni in the mainland of japan. small numbers of h. kitaokai, amblyomma testudinarium and ixodes ovatus were also recorded. | 2002 | 12185317 |
| seroepidemiological study of canine ehrlichial infections in yamaguchi prefecture and surrounding areas of japan. | randomly selected serum samples from 150 dogs from yamaguchi and neighbouring prefectures were subjected to the indirect immunofluorescent assay to detect antibodies against ehrlichia canis, ehrlichia chaffeensis, ehrlichia muris and ehrlichia from ixodes ovatus. a total of 30 out of the 150 serum samples reacted with at least one of the antigens at a titer of 1:20 or more. considerable cross-reactivity was seen and most samples reacted with at least two different antigens. fifteen (10.0%) dogs ... | 2004 | 15350665 |
| detection of dna of 'candidatus mycoplasma haemominutum' and spiroplasma sp. in unfed ticks collected from vegetation in japan. | dna fragments of 'candidatus mycoplasma haemominutum', a feline heamobartonella pathogen, were detected from unfed ixodes ovatus collected from vegetation in hokkaido, fukushima and yamaguchi prefectures, and unfed haemaphysalis flava in yamaguchi prefecture. this finding suggests that ixodid tick is a possible vector of 'c. mycoplasma haemominutum'. spiroplasma dna was also detected from unfed i. ovatus in hokkaido, fukushima and yamaguchi prefectures. the analysis of nucleotides sequence sugge ... | 2005 | 16397391 |