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identification of replication specificity determinants in two strains of tomato leaf curl virus from new delhi.we used two strains of tomato leaf curl virus from new delhi to investigate specificity in replication of their cognate genomes. the strains share 94% sequence identity and are referred to as severe and mild on the basis of symptoms on tomato and tobacco. replication assays in tobacco protoplasts and plants showed that a single amino acid change, asn10 to asp in the n terminus of rep protein, determines specificity for replication of the two strains based upon its interaction with the origin of ...199910364296
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