identification of replication specificity determinants in two strains of tomato leaf curl virus from new delhi. | we used two strains of tomato leaf curl virus from new delhi to investigate specificity in replication of their cognate genomes. the strains share 94% sequence identity and are referred to as severe and mild on the basis of symptoms on tomato and tobacco. replication assays in tobacco protoplasts and plants showed that a single amino acid change, asn10 to asp in the n terminus of rep protein, determines specificity for replication of the two strains based upon its interaction with the origin of ... | 1999 | 10364296 |