Publications

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neoergasilus japonicus (poecilostomatoida: ergasilidae), a parasitic copepod new to north america.adult, gravid female neoergasilus japonicus harada, 1930 are described from several species of fish from lee county, alabama. samples of bluegill lepomis macrochirus rafinesque (n = 25), largemouth bass micropterus salmoides lacépéde (n = 6), redear sunfish lepomis microlophus gunther (n = 5), and channel catfish ictalurus punctatus rafinesque (n = 1) were collected between november 1993 and may 1995. prevalence of infection was 100% in all fish collected. the dorsal fin was the site of infectio ...19989488343
development and use of modified live edwardsiella ictaluri vaccine against enteric septicemia of catfish.the present study showed that e. ictaluri re-33 vaccine does not cause esc but does stimulate protective immunity. the re-33 vaccinates were protected against esc for at least 4 months following a single bath immersion in a low number of e. ictaluri re-33 without booster vaccination. antibody responses are weak after re-33 vaccination. edwardsiella ictaluri re-33 vaccine presents no risk or hazard to catfish. re-33 vaccine will prevent esc caused by most isolates of e. ictaluri in catfish. we re ...19999890040
cyanobacteria and earthy/musty compounds found in commercial catfish (ictalurus punctatus) ponds in the mississippi delta and mississippi--alabama blackland prairie.the compounds responsible for earthy and musty "off-flavors" in farm-raised channel catfish (ictalurus punctatus) in the southeastern united states of america are geosmin and 2-methylisoborneol (mib), respectively. these compounds are produced by certain species of cyanobacteria (blue-green algae) that grow in the aquaculture ponds. previous research has focused on the species of cyanobacteria found in catfish ponds in west mississippi (the leading region of catfish production in the usa), while ...200515993924
an asian origin of virulent aeromonas hydrophila responsible for disease epidemics in united states-farmed catfish.since 2009, catfish farming in the southeastern united states has been severely impacted by a highly virulent and clonal population of aeromonas hydrophila causing motile aeromonas septicemia (mas) in catfish. the possible origin of this newly emerged highly virulent a. hydrophila strain is unknown. in this study, we show using whole-genome sequencing and comparative genomics that a. hydrophila isolates from diseased grass carp in china and catfish in the united states have highly similar genome ...201424895303
rapid quantitative detection of aeromonas hydrophila strains associated with disease outbreaks in catfish aquaculture.a new strain of aeromonas hydrophila has been implicated in significant losses in farm-raised catfish. outbreaks attributable to this new strain began in alabama in the summer of 2009 and have spread to arkansas and mississippi in subsequent years. these outbreaks mostly afflicted market-sized fish and resulted in considerable losses in short periods of time. the present research was designed to develop an expeditious diagnostic procedure to detect the new strains of a. hydrophila due to the rap ...201323847227
the influence of geographical location, host maturity and sex on intestinal helminth communities of the double-crested cormorant phalacrocorax auritus from the eastern united states.here the intestinal helminth infracommunities of 218 double-crested cormorants (phalacrocorax auritus) from 11 locations in alabama, minnesota, mississippi and vermont are documented. trematode infections were present in 98% of hosts; 65% of cormorants carried cestode infections, 4% were infected with acanthocephalans and 66% had nematode intestinal parasites. parasite infracommunities of hosts collected on wintering grounds had higher richness and diversity than did birds collected on breeding ...201627677375
lesions caused by virulent aeromonas hydrophila in farmed catfish ( ictalurus punctatus and i. punctatus × i. furcatus) in mississippi.in 2009, a virulent strain of aeromonas hydrophila caused severe disease and high mortalities (motile aeromonad septicemia variant [masv]) in farmed channel catfish ( ictalurus punctatus) and hybrid catfish ( i. punctatus × i. furcatus) in eastern mississippi and alabama. as is common in mas, there is severe hemorrhagic dermatitis with ulceration, as well as abdominal hyperemia, petechiation, and mild ascites. additional findings in masv cases include panophthalmitis and orbital cellulitis, lead ...201728482758
pcdd, pcdf, and pcb in farm-raised catfish from southeast united states--concentrations, sources, and cyp1a induction.nine catfish fillets, three catfish nuggets, two feed samples, and one pond sediment were analyzed for pcdd, pcdf, and pcb. farm-raised catfish from mississippi, alabama, and arkansas contained significant levels of 2,3,7,8-substituted pcdd and pcdf. in addition, a large number of non-2,3,7,8-substituted congeners were present in all samples. the catfish fillets and catfish nuggets also contained high concentrations of dioxin-like pcb, as well as a number of non-dioxin-like pcb. the teq based on ...20189828294
identification, virulence, and mass spectrometry of toxic ecp fractions of west alabama isolates of aeromonas hydrophila obtained from a 2010 disease outbreak.in west alabama, disease outbreaks in 2009 caused by aeromonas hydrophila have led to an estimated loss of more than $3 million. in 2010, disease outbreak occurred again in west alabama, causing losses of hundreds of thousands of pounds of market size channel catfish. during the 2010 disease outbreak in west alabama, four isolates of a. hydrophila were cultured from the kidney tissues of diseased channel catfish. both analytical profile index (api) 20 e biochemical tests and 16s-23s rrna sequenc ...201323523171
farm size, seining practices, and salt use: risk factors for aeromonas hydrophila outbreaks in farm-raised catfish, alabama, usa.in freshwater aquaculture systems, aeromonas hydrophila is usually considered to be an opportunistic pathogen most often associated with secondary bacterial infections. since 2009, the u.s. catfish industry, especially in west alabama, has been affected by mortality from a strain of a. hydrophila that has been acting as a primary pathogen. tens of millions of pounds of catfish production have been lost as a consequence of this disease. this study used data from two whole-population farmer survey ...201525466218
development and efficacy of novobiocin and rifampicin-resistant aeromonas hydrophila as novel vaccines in channel catfish and nile tilapia.three attenuated aeromonas hydrophila vaccines were developed from the virulent 2009 west alabama isolates through selection for resistance to both novobiocin and rifampicin. when channel catfish (ictalurus punctatus) were ip injected with 4×105 colony-forming unit (cfu) of the mutants, no fish died. however, when the same age and size matched channel catfish were ip injected with similar amount of their virulent parents, 80-100% fish died. similarly, when nile tilapia (oreochromis niloticus) we ...201121872628
identification of unique dna sequences present in highly virulent 2009 alabama isolates of aeromonas hydrophila.in 2009, a disease outbreak caused by aeromonas hydrophila occurred in 48 catfish farms in west alabama, causing an estimated loss of more than 3 million pounds of food size channel catfish. virulence studies have revealed that the 2009 isolates of a. hydrophila are at least 200-fold more virulent than a 1998 alabama isolate al98-c1b. however, up to now, no molecular markers have been identified to differentiate the highly virulent 2009 isolates from other isolates of a. hydrophila. to understan ...201121561726
host-specific association between flavobacterium columnare genomovars and fish species.a total of 90 flavobacterium columnare isolates were recovered from predominant wild fish species in the mobile river, alabama, usa. isolates were identified and confirmed by fatty acid methyl ester analysis and specific pcr amplification. genomovar ascription was performed using 16s-restriction fragment length polymorphism (rflp) analysis. the majority of genomovar i isolates were recovered from threadfin shad while genomovar ii isolates came from catfish (including channel and blue catfish). a ...200717706907
variations in pcb concentrations between genders of six warmwater fish species in lake logan martin, alabama, usa.we collected and analyzed 955 individual fish (six species) for sexual differences in pcb bioaccumulations from a southeastern, usa reservoir. using 2-way ancovas, we found significant differences in fillet pcb concentrations between sexes for channel catfish (ictalurus punctatus), largemouth bass (micropterus salmoides) and spotted bass (micropterus punctulatus). striped bass (morone saxatilus), black crappie (pomoxis nigromaculatus) and freshwater drum (aplodinotus grunniens) did not display d ...200717490714
occurrences and fate of ddt principal isomers/metabolites, dda, and o,p'-ddd enantiomers in fish, sediment and water at a ddt-impacted superfund site.in the 1950s and 60s, discharges from a ddt manufacturing plant contaminated a tributary system of the tennessee river near huntsville, alabama, usa. regulatory action resulted in declaring the area a superfund site which required remediation and extensive monitoring. monitoring data collected from 1988, after remediation, through 2011 showed annual decreases approximating first-order decay in concentrations of total ddt and its six principal congeners (p,p'-ddt, o,p'-ddt, p,p'-ddd, o,p'-ddd, p, ...201425150505
aquaculture genomics, genetics and breeding in the united states: current status, challenges, and priorities for future research.advancing the production efficiency and profitability of aquaculture is dependent upon the ability to utilize a diverse array of genetic resources. the ultimate goals of aquaculture genomics, genetics and breeding research are to enhance aquaculture production efficiency, sustainability, product quality, and profitability in support of the commercial sector and for the benefit of consumers. in order to achieve these goals, it is important to understand the genomic structure and organization of a ...201728219347
molecular identification and virulence of three aeromonas hydrophila isolates cultured from infected channel catfish during a disease outbreak in west alabama (usa) in 2009.three isolates (al09-71, al09-72, and al09-73) of aeromonas hydrophila were cultured from infected channel catfish ictalurus punctatus during a disease outbreak in west alabama, usa, in august 2009. sequence analysis of the 16s-23s rdna intergenic spacer region (isr), cpn60, gyrb, and rpod genes of the 3 strains revealed that the 3 strains were closely related to each other, sharing 97 to 99% nucleotide sequence similarities. however, isr sequences of the 3 isolates from 2009 shared only 64% nuc ...201121790073
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