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detection and enumeration of cryptosporidium sp. oocysts in sewage sludge samples from the city of florianópolis (brazil) by using immunomagnetic separation combined with indirect immunofluorescence assay.in the last few decades, agricultural reclamation of sewage sludge has increased in many countries. however, this practice must be adopted under recommended conditions to limit the risks associated with the presence of potential pathogens, including protozoan parasites such as cryptosporidium. in the present work, we evaluated immunomagnetic separation (ims) combined with an indirect immunofluorescence assay (ifa) in order to detect cryptosporidium oocysts in sewage sludge samples obtained from ...200415253500
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