| molecular identification of sympatric chromosomal forms of anopheles gambiae and further evidence of their reproductive isolation. | three chromosomal forms of anopheles gambiae s.s., designated as bamako, mopti and savanna, were studied for diagnostic pcr assays based on the analysis of the x-linked ribosomal dna (rdna). the study was performed on a 1.3 kb fragment containing part of the 28s coding region and part of the intergenic spacer region. the amplified material was cut with fourteen restriction enzymes to detect restriction fragment length polymorphisms (rflps). the enzymes tru9i and hhai produced patterns of dna ban ... | 1997 | 9359579 |
| mark-release-recapture experiments with anopheles gambiae s.l. in banambani village, mali, to determine population size and structure. | mark-release-recapture experiments with anopheles gambiae s.l. were performed during the wet seasons of 1993 and 1994 in banambani, mali. all recaptured mosquitoes were identified to species by pcr analysis and, when possible, by chromosomal analysis to chromosomal form. two species of the an. gambiae complex were present: an. gambiae s.s. and an. arabiensis; their ratio differed greatly from one year to the next. three chromosomal forms of an. gambiae s.s. were found--bamako, savanna and mopti. ... | 1998 | 9513942 |
| gametocyte infectivity by direct mosquito feeds in an area of seasonal malaria transmission: implications for bancoumana, mali as a transmission-blocking vaccine site. | infectivity of gametocytemic volunteers living in bancoumana, a village 60 km from bamako, mali, was determined by direct feeds of laboratory-reared anopheles gambiae s. l. gametocytemic adolescents (10-18 years old) were as infectious to mosquitoes as younger volunteers and appear to be a more suitable population for testing transmission-blocking efficacy as compared with adults (> 18 years old). to begin to validate the membrane-feeding assay, sera collected from these same volunteers were sub ... | 1998 | 9749648 |
| multilevel analyses of genetic differentiation in anopheles gambiae s.s. reveal patterns of gene flow important for malaria-fighting mosquito projects. | malaria control projects based on the introduction and spread of transgenes into mosquito populations depend on the extent of isolation between those populations. on the basis of the distribution of paracentric inversions, anopheles gambiae has been subdivided into five subspecific chromosomal forms. estimating gene flow between and within these forms of an. gambiae presents a number of challenges. we compared patterns of genetic divergence (f(st)) between sympatric populations of the bamako and ... | 2005 | 15677750 |
| longitudinal survey of knockdown resistance to pyrethroid (kdr) in mali, west africa, and evidence of its emergence in the bamako form of anopheles gambiae s.s. | studies aimed at monitoring the spread of knockdown resistance to pyrethroids (kdr) in time and space are particularly useful for detecting barriers to gene flow among the chromosomal and molecular forms of anopheles gambiae. we used a recently developed polymerase chain reaction assay to estimate changes in kdr frequency that occurred in several mixed-form populations from mali, west africa, in the past decade. we found that the kdr allele significantly increased in frequency in most population ... | 2007 | 17255234 |
| [presence and risk of transmission of wuchereria bancrofti is a reality in rural mali: the case of the town of bariambani in the cirle of kati]. | previous studies on lymphatic filariasis in mali showed high infection rate in rural area. this study was aimed to find wuchereria bancrofti microfilaria in human peripheral blood and larvae in vectors. for that purpose we carried out an entomological, clinical and parasitological study in banambani a village located at 25 km from bamako the capital city. the parasitological and clinical study was a single cross sectional study including night blood thick smear and physical examination. the ento ... | 2006 | 17390523 |
| evaluation and optimization of membrane feeding compared to direct feeding as an assay for infectivity. | malaria parasite infectivity to mosquitoes has been measured in a variety of ways and setting, includind direct feeds of and/or membrane feeding blood collected from randomly selected or gametocytemic volunteers. anopheles gambiae s.l is the main vector responsible of plasmodium falciparum transmission in bancoumana and represents about 90% of the laboratory findings, whereas plasmodium malariae and plasmodium ovale together represent only 10%. | 2008 | 19055715 |