| comparison of genetic characteristics and pathogenicity of lactococcus garvieae isolated from aquatic animals in taiwan. | seventy-six taiwanese bacterial isolates including 74 from diseased, cultured, aquatic animals (54 grey mullet mugil cephalus, 3 basket mullet chelon alatus, 2 tilapia oreochromis niloticus, 1 grouper epinephelus coioides, 2 yellowfin seabream acanthopagrus latus, 1 borneo mullet chelon macrolepis, 1 bullfrog rana catesbeiana, 1 japanese eel anguilla japonica, and 9 giant freshwater prawns macrobrachium rosenbergii), 1 wild-caught seafood species (squid muscle collected from a restaurant) and 1 ... | 2012 | 23209077 |
| macrobrachium rosenbergii nodavirus infection in m. rosenbergii (de man) with white tail disease cultured in taiwan. | white tail disease (wtd) is a serious problem in macrobrachium rosenbergii hatcheries and nursery ponds in asia. the causative agents have been identified as m. rosenbergii nodavirus (mrnv) and its associated extra small virus. this is the first report demonstrating mrnv virus in m. rosenbergii displaying wtd signs in taiwan by reverse transcriptase-polymerase chain reaction (rt-pcr). amplified fragments of 850 and 425 bp for rna-1 and rna-2 of mrnv, respectively, were obtained by rt-pcr. rt-pcr ... | 2008 | 18471097 |
| metschnikowia bicuspidata dominates in taiwanese cold-weather yeast infections of macrobrachium rosenbergii. | at water temperatures below 17 degrees c, yeast infections often occurred in 6 to 11 mo old giant freshwater prawn macrobrachium rosenbergii (8 to 12 cm) in taiwan from may 2001 to december 2003, with a cumulative mortality of 20 to 95%. gross signs of disease included milky hemolymph, a yellow exoskeleton, opaque muscles, and a swollen hepatopancreas (hp). histopathology included marked edema and extensive necrotic lesions associated with large numbers of yeast aggregates and inflammation withi ... | 2007 | 17629113 |
| rt-pcr amplification and sequence analysis of extra small virus associated with white tail disease of macrobrachium rosenbergii (de man) cultured in taiwan. | post-larvae of macrobrachium rosenbergii infected with white tail disease (wtd) have been reported in taiwan. the causative agents have been identified as m. rosenbergii nodavirus (mrnv) associated with extra small virus (xsv). the present study is the first report confirming the presence of xsv virus in m. rosenbergii displaying wtd symptoms in taiwan by reverse transcription polymerase chain reaction (rt-pcr). a 772 bp amplified product was obtained by rt-pcr, cloned and sequenced. the nucleot ... | 2007 | 17352787 |
| in situ hybridization and rt-pcr detection of macrobrachium rosenbergii nodavirus in giant freshwater prawn, macrobrachium rosenbergii (de man), in taiwan. | between january and march 2006, suspected outbreaks of white tail disease were observed in post-larvae and juveniles of macrobrachium rosenbergii in hatcheries and nursery ponds at kaohsiung and pingtung counties in southern taiwan. pathognomonic lesions showed the presence of large oval and/or irregular basophilic, cytoplasmic inclusion bodies in the infected muscles and hepatopancreas. reverse transcriptase polymerase chain reaction (rt-pcr) assay and in situ hybridization (ish) revealed evide ... | 2006 | 17169113 |
| engulfed pathogen-induced apoptosis in haemocytes of giant freshwater prawn, macrobrachium rosenbergii. | haemocytes of the giant freshwater prawn, macrobrachium rosenbergii, were investigated for the induction of apoptosis after phagocytosis of pathogenic yeasts, bacteria and non-pathogenic latex beads in vitro. isolated haemocytes of m. rosenbergii were cultured at a ratio of 1:50 haemocytes to pathogen with the yeast debaryomyces hansenii, the bacteria aeromonas hydrophila or enterococcus faecium, or with latex beads at 25 degrees c for 2 h, followed by washing to remove free particles. at least ... | 2005 | 16336474 |
| lactococcus garvieae infection in the giant freshwater prawn macrobranchium rosenbergii confirmed by polymerase chain reaction and 16s rdna sequencing. | an epizootic bacterial infection in the giant freshwater prawn macrobranchium rosenbergii occurred in taiwan from may to june 1999. the cumulative mortality was approximately 30 to 75%. the diseased prawns showed opaque and whitish muscles and were approximately 2 mo old with total lengths from 5 to 6 cm. histopathologically, they showed marked edema and necrotic lesions with inflammation in the muscles and hepatopancreas. bacteria isolated using brain heart infusion medium or tryptic soy agar w ... | 2001 | 11411643 |
| isolation and characterization of an enterococcus-like bacterium causing muscle necrosis and mortality in macrobrachium rosenbergii in taiwan. | a gram-positive, ovoid, diplococoid bacterium tentatively identified as enterococcus-like was isolated from diseased macrobrachium rosenbergii in taiwanese aquaculture ponds. the diseased prawns displayed poor growth, anorexia, inactivity, opaque and whitish musculature, and mortality. in histological preparations, melanized hemocytic granulomas were seen in the connective tissue around hemal sinuses together with hemocytic aggregation in necrotic musculature. five isolates of diplococci were co ... | 1998 | 9828405 |