| field application of polymerase chain reaction diagnosis and strain typing of trypanosoma cruzi in bolivian triatomines. | a new approach for direct identification and characterization of trypanosoma cruzi stocks in biological samples was tested for field applicability on an extensive sample of feces collected from triatomine vectors from four different species found in bolivia. the first step of the technique is polymerase chain reaction (pcr) amplification of the hypervariable region of kinetoplast dna minicircles of t. cruzi parasites. in this report, 345 fecal samples were analyzed and the pcr results were compa ... | 1995 | 7677221 |
| smallness of the panmictic unit of triatoma infestans (hemiptera: reduviidae). | the population genetic structure of triatoma infestans (klug), the principal vector of the causative agent of chagas disease in bolivia, was investigated by enzyme electrophoresis at 15 loci, of which 3 were polymorphic. a total of 1,286 adults and nymphs was collected from 19 localities of the cochabamba (high endemicity) and la paz (low endemicity) departments. previous results were confirmed, including a low level of polymorphism (0.20), low genetic distance between geographic areas, and a po ... | 1998 | 9835679 |
| mitochondrial dna variation of triatoma infestans populations and its implication on the specific status of t. melanosoma. | dna sequence comparison of 412 base-pairs fragments of the mitochondrial cytochrome b gene was used to infer the genetic structure of nine geographical triatoma infestans populations and their phylogenetic relationship with t. melanosoma and t. brasiliensis. t. infestans and t. melanosoma were compared by morphometry, allozyme and cytogenetic analyses, as well as subjected to reciprocal crosses, in order to clarify the taxonomic status of the latter. no differences were found to distinguish the ... | 1999 | 10677723 |
| domiciliation process of rhodnius stali (hemiptera: reduviidae) in alto beni, la paz, bolivia. | we report a systematic collection of triatominae inside houses and in the peridomestic environment of alto beni, department of la paz, bolivia. this area is free of triatoma infestans and although we detected previously seropositivity for trypanosoma cruzi, the alto beni region is not officially considered as endemic for chagas disease. from 11 houses of five localities, we collected adults, nymphs and eggs of a rhodnius species, which was confirmed by morphological and morphometric analysis as ... | 2003 | 12631318 |
| active dispersal by wild triatoma infestans in the bolivian andes. | triatoma infestans is the main vector of chagas disease and target of control programmes in the southern cone countries. so far bolivia is the only country where true t. infestans wild foci are documented. the dispersal ability for wild t. infestans was studied at microgeographical scale in bolivian andes, to assess the possibility for wild populations to actively recolonize insecticide-treated villages. nine microsatellite loci were used to detect the extent of gene flow between neighbouring co ... | 2007 | 17550473 |
| wild triatoma infestans, a potential threat that needs to be monitored. | the current persistence of triatoma infestans, and therefore of chagas disease transmission, in the andean valleys of bolivia and the gran chaco (precisely where wild populations of the vector are widespread), indicates a possible relationship between these two occurrences. this paper provides an overview of the current knowledge regarding wild t. infestans in bolivia. the different morphs of the wild vector, their known distributions and some traits of their biology and ecology are presented. p ... | 2009 | 19753459 |
| first report of widespread wild populations of triatoma infestans (reduviidae, triatominae) in the valleys of la paz, bolivia. | wild populations of triatoma infestans, the main vector of chagas disease in the southern cone countries, may be involved in reinfestation of human dwellings, limiting the success of vector-control campaigns in bolivia. knowledge of the distribution of these populations remains incomplete. we report here the detection of t. infestans wild populations in large areas in the department of la paz, bolivia. among 18 sylvatic areas investigated, 17 were positive with t. infestans specimens. the infect ... | 2010 | 20348501 |