| survey of ovine and caprine toxoplasmosis by ifat and pcr assays in sardinia, italy. | during the period 1999-2002, we have analyzed 9639 serum samples and 815 aborted samples (670 fetuses and 145 placenta) from 964 ovine and caprine farms distributed over all sardinia island. after abortion notification, sera collected at random from adult animals were examined to detect simultaneously igg and igm antibodies specific to toxoplasma gondii by indirect immunofluorescence assay, whereas fetuses and placenta were analyzed by a single tube nested pcr assay. specific igg antibodies were ... | 2003 | 14597274 |
| detection of pathogens in ovine and caprine abortion samples from sardinia, italy, by pcr. | during 2003-2005, 399 abortion samples (315 fetuses and 84 placentae) were collected from 107 ovine and caprine farms in northern sardinia. tissues from aborted fetuses and placentae were examined by pcr assay to detect dna from coxiella burnetii, chlamydophila abortus, salmonella enterica serovar abortusovis, toxoplasma gondii, and neospora caninum. the dna from at least 1 of these 5 infectious agents was amplified in 41% of ovine fetuses, while only 17% of the caprine fetuses yielded a positiv ... | 2007 | 17459840 |
| molecular characterization of toxoplasma gondii type ii in sheep abortion in sardinia, italy. | during 2009-2010, 161 tissue samples (142 placentas, 16 brains, and 3 livers) from aborted ovine fetuses on sardinia island, italy, were tested for toxoplasmosis. organs that showed a positive result by nested polymerase chain reaction (pcr) targeting the its1 region for toxoplasma gondii were also amplified with 11 genetic markers (sag1, 5'-sag2, 3'-sag2, sag3, btub, gra6, c22-8, c29-2, l358, pk1, and apico) and then subjected to pcr/rflp for genetic typing. t. gondii dna was found in 5 placent ... | 2014 | 24534616 |
| sero-epidemiological update on sheep toxoplasmosis in sardinia, italy. | in order to better understand the epidemiology of ovine toxoplasmosis in sardinia, a serological survey was carried out on 22 flocks with no fertility problems. in total 1043 sera (9% of the 11,382 sheep raised in the flocks) were examined by means of a commercial elisa kit. to verify the performance of elisa test, 160 selected sera were tested again with a gold standard test (ifat). performance of the commercial elisa kit was summarised in terms of sensitivity (se), specificity (sp), positive a ... | 2007 | 18689234 |
| prevalence of neospora caninum infection in sardinian dairy farms (italy) detected by iscom elisa on tank bulk milk. | neospora caninum is a heteroxenous cyst-forming coccidian closely related to toxoplasma gondii and is considered one of the major causes of abortions in cattle worldwide. the present work aims to update the epidemiological trend of n. caninum of dairy cattle in sardinia island, western mediterranean (italy). for this reason, we used the newest enzyme-linked immunoassay (elisa) methodology that exploits immune-stimulating complexes (iscoms) principle and allows us to point out the infection in th ... | 2006 | 16323023 |