cerdocyon thous (l.) (carnivora, canidae) naturally infected with leishmania donovani chagasi (cunha & chagas, 1973) in corumbá (mato grosso do sul state, brazil). | | 1988 | 2593825 |
[occurrence of canine visceral leishmaniasis in an agricultural settlement in the state of mato grosso do sul, brazil]. | during previous research on phlebotomine fauna in a settlement of the brazilian national agrarian reform institute (incra) on the bodoquena range, mato grosso do sul state, brazil, dogs were observed with clinical aspects suggestive of visceral leishmaniasis. a serological survey to leishmaniasis in 97 dogs, by indirect immunofluorescence test, showed 23 (23.7%) serum positive dogs. samples of the parasites were identified as leishmania (leishmania) chagasi. | 2001 | 11460220 |
isolation and identification of 9-methylgermacrene-b as the putative sex pheromone of lutzomyia cruzi (mangabeira, 1938) (diptera: psychodidae). | lutzomyia (lutzomyia) cruzi has been named as a probable vector of leishmania chagasi in corumbá, mato grosso do sul, brazil. taxonomically l. cruzi is closely related to the l. longipalpis species complex. females of l. cruzi and l. longipalpis are morphologically indistinguishable and associated males must be examined carefully to confirm identifications. chemical analysis hexane extracts of male l. cruzi has revealed the presence of a 9-methylgermacrene-b (c16), a homosesquiterpene (mw 218) p ... | 2002 | 12048580 |
occurrence of neospora caninum in dogs and its correlation with visceral leishmaniasis in the urban area of campo grande, mato grosso do sul, brazil. | neospora caninum is an obligate intracellular protozoan that can infect domestic and wild canids, as well as ruminants and equines, and is described as causing neuromuscular alteration and death in dogs. visceral leishmaniasis (vl) is an infectious disease that affects both humans and animals, being caused by protozoan parasites of the genus leishmania, of which leishmania (leishmania) chagasi is found in brazil -- transmitted by sand flies, such as lutzomyia longipalpis, in most of the american ... | 2006 | 16310954 |
occurrence of co-infection by leishmania (leishmania) chagasi and trypanosoma (trypanozoon) evansi in a dog in the state of mato grosso do sul, brazil. | a natural case of co-infection by leishmania and trypanosoma is reported in a dog (canis familiaris) in south- western state of mato grosso do sul, brazil. both amastigote and trypomastigote forms were observed after giemsa staining of cytological preparations of the dog's bone marrow aspirate. no parasite was detected using medium culture inoculation of the sample. dna obtained from the bone marrow aspirate sample and from the blood buffy coat was submitted to polymerase chain reaction (pcr) wi ... | 2006 | 16410962 |
natural infection of phlebotomines (diptera: psychodidae) in a visceral-leishmaniasis focus in mato grosso do sul, brazil. | the main purpose of this study was to investigate natural infection by leishmania in phlebotomine females in a visceral-leishmaniasis focus in antonio joão county in mato grosso do sul state, brazil. between june and october 2003, the digestive tracts of 81 females captured in aldeia campestre, aldeia marangatu and povoado campestre were dissected. the females were separated by species, location, area and date of capture into 13 groups and kept in ethanol 70%. to identify the leishmania species ... | 2007 | 17505673 |
first report of natural infection of a bush dog (speothos venaticus) with leishmania (leishmania) chagasi in brazil. | we report here the first known case of natural infection of a bush dog with leishmania (leishmania) chagasi in brazil. the specimen was captured in the wild in the state of mato grosso and is currently being held in captivity at fundação jardim zoológico, rio de janeiro, brazil. the leishmaniasis was diagnosed by culture of promastigote forms in intact skin fragments and their characterization by isoenzyme electrophoresis. this report calls attention to the parasitological and etiological contro ... | 2008 | 18036627 |
detection of natural infection in lutzomyia cruzi and lutzomyia forattinii (diptera: psychodidae: phlebotominae) by leishmania infantum chagasi in an endemic area of visceral leishmaniasis in brazil using a pcr multiplex assay. | in order to identify lutzomyia spp. naturally infected by leishmania parasites a pcr multiplex assay coupled to non-isotopic hybridization was used for the analysis of insect samples collected by cdc light traps in an endemic area of visceral leishmaniasis (vl) in the municipality of corumbá, mato grosso do sul state, brazil in may/june 2006. wild sand flies were identified and grouped into pools of 10 female specimens and 27 groups in total were collected. positive results were obtained from lu ... | 2008 | 18502392 |
tesa-blot for the diagnosis of chagas disease in dogs from co-endemic regions for trypanosoma cruzi, trypanosoma evansi and leishmania chagasi. | we standardized serodiagnosis of dogs infected with trypanosoma cruzi using tesa (trypomastigote excreted-secreted antigen)-blot developed for human chagas disease. tesa-blot showed 100% sensitivity and specificity. in contrast, elisa using tesa (tesa-elisa) or epimastigotes (epi-elisa) as antigen yielded 100% sensitivity but specificity of 94.1% and 49.4%, respectively. when used in field studies in an endemic region for chagas disease, visceral leishmaniasis and trypanosoma evansi (mato grosso ... | 2009 | 19426657 |
[identification of leishmania species isolated in human cases in mato grosso do sul, by means of the polymerase chain reaction]. | leishmaniases are endemic zoonoses in the state of mato grosso do sul. their etiological agents in this region of brazil are leishmania (leishmania) chagasi, leishmania (leishmania) amazonensis and leishmania (viannia) braziliensis. the polymerase chain reaction (pcr) is a tool with high specificity and sensitivity for identifying leishmania species. this study examined 39 cryopreserved isolates of leishmania that had been collected by bone marrow aspiration and/or lesion biopsy, depending on th ... | 2009 | 19684979 |
leishmania(leishmania) chagasi in captive wild felids in brazil. | this study used a pcr-rflp test to determine the presence of leishmania (leishmania) chagasi in 16 captive wild felids [seven puma concolor (linnaeus, 1771); five panthera onca (linnaeus, 1758) and four leopardus pardalis (linnaeus, 1758)] at the zoological park of the federal university of mato grosso, brazil. amplification of leishmania spp. dna was seen in samples from five pumas and one jaguar, and the species was characterized as l. chagasi using restriction enzymes. it is already known tha ... | 2010 | 19740501 |
phlebotomine fauna (diptera: psychodidae) of an american cutaneous leishmaniasis endemic area in the state of mato grosso do sul, brazil. | the occurrence of an outbreak of cutaneous leishmaniasis associated with leishmania (leishmania) amazonensis in the municipality of bela vista, state of mato grosso do sul, brazil, and the absence of information on its vectors in this area led the authors to undertake captures of phlebotomine sand flies, using shannon traps and automatic cdc light traps, in domiciles, forested areas and animal shelters from february 2004-january 2006. a total of 808 specimens belonging to 18 sandfly species have ... | 2009 | 19820827 |
leishmania (leishmania) infantum/chagasi: histopathological aspects of the skin in naturally infected dogs in two endemic areas. | in the new world, visceral leishmaniasis (vl), which is a progressive disease and frequently fatal, is caused by leishmania (leishmania) infantum/chagasi. it is endemic in many regions of brazil and occasionally occurs in non-endemic regions when dogs from an endemic area are introduced. the aim of the present study is to compare different skin infection patterns of dogs from two leishmaniasis endemic areas. a histological analysis of dogs from campo grande, mato grosso do sul state, a region wh ... | 2010 | 19854175 |
[leishmania (leishmania) infantum chagasi in wild canids kept in captivity in the state of mato grosso]. | visceral leishmaniasis is a zoonosis that affects many mammals, and domestic canids are the main reservoirs in urban environments. this note describes infection by leishmania (leishmania) infantum chagasi among wild canids kept in captivity in the state of mato grosso, brazil. | 2010 | 20563507 |
[ecological aspects of phlebotomines (diptera: psychodidae) in the urban area of ponta porã municipality, state of mato grosso do sul, brazil]. | the aim of this study was to carry out an analysis of urban phlebotomine fauna and a survey of infestations (intradomiciliary and peridomiciliary) in ponta porã municipality state of mato grosso do sul. | 2010 | 21181032 |
[evidence of transmission of visceral leishmaniasis by lutzomyia cruzi in the municipality of jaciara, state of mato grosso, brazil]. | the municipality of jaciara was classified in 2003 as a transmission area for visceral leishmaniasis in outbreak situations. this study aimed to establish evidence of transmission of leishmania (leishmania) infantum chagasi by lutzomyia cruzi in the municipality of jaciara, state of mato grosso, brazil. | 2011 | 21340413 |
use of parasitological culture to detect leishmania (leishmania) chagasi in naturally infected dogs. | in brazil, although the domestic dog is a major target for the control actions for visceral leishmaniasis, knowledge gaps of the leishmania species present in those animals still exist in many endemic areas. the objective of this study was the use of parasitological culture as a diagnosis tool and identification of species of leishmania and other trypanosomatids in the canine population in the city of cuiaba/mato grosso. biological samples such as blood, intact skin fragments, cutaneous ulcers, ... | 2011 | 21919725 |
leishmania chagasi in opossums (didelphis albiventris) in an urban area endemic for visceral leishmaniasis, campo grande, mato grosso do sul, brazil. | we investigated the occurrence of leishmania infantum chagasi in didelphis albiventris opossums at a wild animal rehabilitation center in the city of campo grande, brazil. a total of 54 opossums were tested for l. i. chagasi infection in peripheral blood and bone marrow samples. the samples were analyzed by direct examination, culturing in a specific medium, and polymerase chain reaction-restriction fragment length polymorphism. leishmania i. chagasi dna was detected by polymerase chain reaction ... | 2012 | 22802435 |
molecular and serological detection of tick-borne pathogens in dogs from an area endemic for leishmania infantum in mato grosso do sul, brazil. | tick-borne pathogens affect a wide range of vertebrate hosts. to identify tick-borne pathogens among dogs from campo grande, ms, brazil testing seropositive for leishmania infantum (syn. l. chagasi), a serological and molecular study was conducted to detect ehrlichia canis, anaplasma platys and babesia vogeli in 60 serum and spleen samples. a confirmatory diagnosis of l. infantum based on serological and molecular assays was also performed, as was sequence alignment and phylogenetic analysis to ... | 2014 | 24473877 |
behavioral aspects of lutzomyia longipalpis (diptera: psychodidae) in urban area endemic for visceral leishmaniasis. | the study of some of the behavioral aspects of the main vector of leishmania infantum chagasi cunha & chagas in the americas, lutzomyia longipalpis (lutz & neiva), such as dispersion, population size, and vector survival rates, is important for the elucidation of the mechanisms of visceral leishmaniasis transmission. these parameters were studied by means of capture-mark-release-recapture experiments in an urban area of campo grande municipality, an endemic area of visceral leishmaniasis, situat ... | 2013 | 23540114 |