| the first record in the americas of an autochthonous case of leishmania (leishmania) infantum chagasi in a domestic cat (felix catus) from cotia county, são paulo state, brazil. | a case of feline cutaneous leishmaniasis is reported in a domestic cat (felis catus) as an apparently natural infection in a non-endemic area. amastigotes were seem in smears of a nodular lesion on the cat's nose. no parasite could be seen in cytological preparations of liver or spleen but dna obtained from a sample of the spleen produced the expected fragment in a leishmania specific rdna based pcr assay. the pcr product, a 520 bp fragment, was sequenced and the nucleotide sequence was identica ... | 2004 | 15041097 |
| surveillance for zoonotic vector-borne infections using sick dogs from southeastern brazil. | for many vector-borne organisms, dogs can be used as sentinels to estimate the risk of human infection. the objective of this study was to use dogs as sentinels for multiple vector-borne organisms in order to evaluate the potential for human infection with these agents in southeastern brazil. blood from 198 sick dogs with clinicopathological abnormalities consistent with tick-borne infections were selected at the são paulo state university veterinary teaching hospital in botucatu and tested for ... | 2007 | 18021025 |
| tesa-blot for the diagnosis of chagas disease in dogs from co-endemic regions for trypanosoma cruzi, trypanosoma evansi and leishmania chagasi. | we standardized serodiagnosis of dogs infected with trypanosoma cruzi using tesa (trypomastigote excreted-secreted antigen)-blot developed for human chagas disease. tesa-blot showed 100% sensitivity and specificity. in contrast, elisa using tesa (tesa-elisa) or epimastigotes (epi-elisa) as antigen yielded 100% sensitivity but specificity of 94.1% and 49.4%, respectively. when used in field studies in an endemic region for chagas disease, visceral leishmaniasis and trypanosoma evansi (mato grosso ... | 2009 | 19426657 |
| detection of leishmania (leishmania) amazonensis and leishmania (leishmania) infantum chagasi in brazilian bats. | although bats are one of the most abundant mammals in the new world and are present in virtually all ecosystems, including urban and peri-urban environments, few studies have investigated the role of these animals in the epidemiological chain of leishmaniosis. here, we report a study of 683 bats captured in são paulo county (southeastern from brazil), which were screened by serology, parasitologic methods and polymerase chain reaction (pcr) for trypanosomatids. the indirect immunofluorescent ant ... | 2010 | 19939568 |
| diminished cd4+/cd25+ t cell and increased ifn-gamma levels occur in dogs vaccinated with leishmune in an endemic area for visceral leishmaniasis. | the leishmune vaccine has been used in endemic areas to prevent canine visceral leishmaniasis in brazil, but cytokine production induced by vaccination has rarely been investigated in dogs. this study aimed to evaluate the immune response of dogs vaccinated with leishmune fml vaccine (fort dodge) against total antigen of leishmania (leishmania) chagasi (tag) and fml. twenty healthy dogs from araçatuba, são paulo, brazil, an endemic leishmaniasis area, received three consecutive subcutaneous inje ... | 2010 | 20132994 |
| study of sand fly fauna in an endemic area of american cutaneous leishmaniasis and canine visceral leishmaniasis in the municipality of espírito santo do pinhal, são paulo, brazil. | canine american visceral leishmaniasis and american cutaneous leishmaniasis (acl) cases have been recorded in espírito santo do pinhal. the aim of this study was to gather knowledge of the sand fly community and its population ecology within the municipality. captures were made weekly over a period of 15 months in the urban, periurban and rural areas of the municipality, using automatic light traps. a total of 5,562 sand flies were collected, comprising 17 species. the most abundant species were ... | 2010 | 20428683 |
| occurrence of leishmania (leishmania) chagasi in a domestic cat (felis catus) in andradina, são paulo, brazil: case report. | this work describes natural infection by leishmania in a domestic cat where amastigote forms of the parasite were observed in the popliteal lymph node imprint. positive and negative serological reactions were observed by enzyme-linked immunosorbent assay (elisa) and indirect immunofluorescence assay (ifa), respectively. polymerase chain reaction (pcr) revealed that the nucleotide sequence of the sample was identical to leishmania (l.) chagasi. this is the first report of the disease in felines o ... | 2010 | 21184705 |
| use of crude, fml and rk39 antigens in elisa to detect anti-leishmania spp. antibodies in felis catus. | visceral leishmaniasis is a disease caused by leishmania (leishmania) chagasi and represents a serious public health problem. the dog is the main urban reservoir of the disease; however, investigations regarding the occurrence and epidemiological importance of leishmaniasis in cats have recently been initiated. this study aimed to detect cats seropositive for leishmania spp. using different antigens. additional studies were performed using sera from cats with toxoplasma gondii (n=15) to evaluate ... | 2010 | 21195550 |
| first occurrence of an autochthonous canine case of leishmania (leishmania) infantum chagasi in the municipality of campinas, state of são paulo, brazil. | an autochthonous case of visceral leishmaniasis is reported in a dog (canis familiaris) as an apparently natural infection in a non-endemic area. dna obtained from spleen and liver samples produced the expected fragment in a leishmania-specific rdna-based nested-pcr assay. the pcr product, a 490 bp fragment, was sequenced and the nucleotide sequence was identical to that of leishmania (leishmania) infantum chagasi. these results are surprising since no autochthonous human or canine cases of visc ... | 2011 | 21915468 |
| molecular and serological detection of leishmania spp. in captive wild animals from ilha solteira, sp, brazil. | leishmaniasis is a zoonotic disease that affects 12 million people worldwide. several mammalian species can serve as a reservoir for this disease. dogs are the main reservoir for visceral leishmaniasis in urban areas, which has become a serious public health concern in brazil. the aim of this study was to evaluate the presence of leishmania spp. in captive wild animals from ilha solteira, são paulo, brazil. blood and various tissues samples were collected from animals of five different species: ... | 2011 | 21961752 |
| larval breeding sites of lutzomyia longipalpis (diptera: psychodidae) in visceral leishmaniasis endemic urban areas in southeastern brazil. | the scarcity of information on the immature stages of sand flies and their preferred breeding sites has resulted in the focus of vectorial control on the adult stage using residual insecticide house-spraying. this strategy, along with the treatment of human cases and the euthanasia of infected dogs, has proven inefficient and visceral leishmaniasis continues to expand in brazil. identifying the breeding sites of sand flies is essential to the understanding of the vector's population dynamic and ... | 2013 | 24069494 |
| serological evidence of infection by leishmania (leishmania) infantum (synonym: leishmania (leishmania) chagasi) in free-ranging wild mammals in a nonendemic region of the state of são paulo, brazil. | concerns about the interface between wildlife, domestic animals, and humans in the transmission of visceral leishmaniasis (vl) have been growing due to natural or anthropogenic environmental changes. in this context, investigations of the infection in wild mammals are important to assess their exposure to the vector and the parasite. a study of anti-leishmania (leishmania) infantum antibodies was carried out using the direct agglutination test (dat) on 528 free-ranging wild mammals of 38 species ... | 2015 | 26418884 |
| the first canine visceral leishmaniasis outbreak in campinas, state of são paulo southeastern brazil. | early detection of american visceral leishmaniasis (avl) outbreak in animals is crucial for controlling this disease in non-endemic areas. | 2014 | 25075492 |
| canine visceral leishmaniasis and chagas disease among dogs in araguaína, tocantins. | the present study analyzed serum samples from 111 male and female dogs of various ages from the municipality of araguaína in the state of tocantins, brazil. serological diagnosis of canine visceral leishmaniasis (cvl) was initially performed at the central laboratory (laboratório central - lacen) of araguaína, resulting in 61 positive samples by an indirect immunofluorescence assay (iifa) (≥1:40) and 50 non-reactive samples. the same samples were analyzed at the são paulo institute of tropical m ... | 2013 | 23802237 |