herd prevalence and geographic distribution of, and risk factors for, bovine paratuberculosis in wisconsin.a random sample of wisconsin dairy herds, stratified by herd size, were tested for paratuberculosis by use of an absorbed elisa procedure. the elisa was optimized for overall accuracy by means of receiver operating characteristic curve analysis, and had a sensitivity and specificity of 50.9 and 94.9%, respectively. herd prevalence was analyzed for correlation with responses to a management practices questionnaire completed by the herd owners. one hundred and fifty-eight herds and 4,990 cattle we ...19948163422
epizootic of paratuberculosis in farmed elk.after multiple cases of chronic diarrhea and weight loss in a farmed elk herd, 3 yearlings and 1 adult elk with similar clinical signs were euthanatized and necropsied. gross and histologic evidence of paratuberculosis were found in the yearlings. evidence of serum antibody to mycobacterium paratuberculosis was detected in the 2 elk with the most disseminated infection. acid-fast organisms were isolated from multiple organs in all 4 elk and identified as m paratuberculosis by dna probe. thirty-f ...19989810391
sensitivity of test strategies used in the voluntary johne's disease herd status program for detection of mycobacterium paratuberculosis infection in dairy cattle evaluate sensitivities at the herd level of test strategies used in the voluntary johne's disease herd status program (vjdhsp) and alternative test strategies for detecting dairy cattle herds infected with mycobacterium paratuberculosis.200212420786
detection of mycobacterium avium subspecies paratuberculosis genetic components in retail cheese curds purchased in wisconsin and minnesota by pcr.research has been focused on the detection of mycobacterium avium subspecies paratuberculosis (map) in pasteurized milk; however, pasteurized milk is a key ingredient in a variety of food products. therefore, map contamination in milk-derived products must be investigated. we undertook a six-month study to investigate the presence of viable map and map genetic components in cheese curds purchased from retail outlets in the northern and southern regions of wisconsin and minnesota. a total of 98 r ...200616545540
mycobacterium avium subsp. paratuberculosis in scavenging mammals in wisconsin.the presence of mycobacterium avium subsp. paratuberculosis (map) in non-ruminant wildlife has raised questions regarding the role of these species in johne's disease transmission. in this study we tested 472 tissues from 212 animals of six different species of scavenging mammals. all animals were taken from within a 210-square-mile area in dane and iowa counties of south central wisconsin from september to may in 2003-04 and tested for the presence of map. we detected map-specific dna in 81 of ...200717495318
efficacy of feeding plasma-derived commercial colostrum replacer for the prevention of transmission of mycobacterium avium subsp paratuberculosis in holstein estimate the relative risk of paratuberculosis (johne's disease [jd]) in calves fed a plasma-derived colostrum-replacement (cr) product versus raw bovine maternal colostrum (mc).200919405889
Evaluation of the risk of paratuberculosis in adult cows fed Mycobacterium avium subsp paratuberculosis DNA-positive or -negative colostrum as calves.To estimate the risk of subclinical Mycobacterium avium subsp paratuberculosis (MAP) infection in cows that ingested MAP DNA-positive raw colostrum as calves, compared with risk in cows that ingested MAP DNA-negative raw colostrum as calves.201122023123
epidemiologic study of on-farm management practices associated with prevalence of mycobacterium paratuberculosis infections in dairy use an on-farm recording form to quantity the effect of specific management practices on apparent prevalence of mycobacterium paratuberculosis in dairy cattle herds.19968675478
accidental self-inoculation with mycobacterium paratuberculosis bacterin (johne's bacterin) by veterinarians in wisconsin.we surveyed wisconsin veterinarians to assess the frequency and severity of accidental self- and other human exposure to mycobacterium paratuberculosis bacterin (johne's bacterin). of 199 veterinarians administering the bacterin to cattle, 22 reported one or more exposures, including 19 needle-stick exposures, 8 skin surface exposures, and 2 oral mucosa exposures. the mean incidence of needle sticks was 5.5/100 veterinarians/year of bacterin use or 1/1,000 doses administered. the mean total dose ...19883391850
detection of viable mycobacterium avium subsp. paratuberculosis in retail pasteurized whole milk by two culture methods and pcr.cattle with johne's disease can shed live mycobacterium avium subsp. paratuberculosis (map) in their milk, and map can survive under simulated commercial pasteurization conditions. in several studies conducted in the united kingdom and canada, map dna has been detected in retail pasteurized milk samples; however, in one study in the united kingdom viable map was identified in commercially pasteurized milk. a double-blind study involving two laboratories was undertaken to evaluate retail pasteuri ...200515895728
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