culture phenotypes of genomically and geographically diverse mycobacterium avium subsp. paratuberculosis isolates from different hosts.mycobacterium avium subsp. paratuberculosis causes paratuberculosis (johne's disease) in ruminants in most countries. historical data suggest substantial differences in culturability of m. avium subsp. paratuberculosis isolates from small ruminants and cattle; however, a systematic comparison of culture media and isolates from different countries and hosts has not been undertaken. here, 35 field isolates from the united states, spain, northern ireland, and australia were propagated in bactec 12b ...201121430104
occurrence of mycobacterium avium subsp. paratuberculosis in untreated water in northern ireland.mycobacterium avium subsp. paratuberculosis is the known cause of johne's disease of both domestic and wild ruminants and has been implicated as a possible cause of crohn's disease in humans. the organism is shed in the feces of infected animals and can survive for protracted periods in the environment and hence could be present in catchment areas receiving agricultural runoff. a limited survey was undertaken in northern ireland to test for m. avium subsp. paratuberculosis in untreated water ent ...200516269747
molecular analyses of mycobacteria other than the m. tuberculosis complex isolated from northern ireland cattle.mycobacteria other than the mycobacterium tuberculosis complex (mott), isolated from northern ireland cattle, were identified by pcr amplification of the 16s rrna gene, and subsequent reverse cross blot hybridisation and sequence analyses. elucidation of the mott species was to facilitate specificity testing of new and existing diagnostic test reagents for bovine tuberculosis. the presence of the genes for potential diagnostic antigens: mpb70, mpb64, esat-6 and cfp-10 in the isolated mott specie ...200515917138
incidence of mycobacterium paratuberculosis in raw sheep and goats' milk in england, wales and northern ireland.a blind survey of 104 raw sheep and goats' milk samples (90 goat, 14 sheeps) from bulk tanks on farms throughout england, wales and northern ireland was carried out over a 5-month period (january-may 1998) in order to determine the incidence of mycobacterium paratuberculosis. each milk sample (100 ml) was divided into two 50ml portions. one portion was decontaminated with 0.75% hexadecylpyridinium chloride for 5h before culture on slopes of herrold's egg yolk medium and in bactec radiometric med ...200111230934
mycobacterium avium subsp. paratuberculosis in lake catchments, in river water abstracted for domestic use, and in effluent from domestic sewage treatment works: diverse opportunities for environmental cycling and human exposure.mycobacterium avium subsp. paratuberculosis from infected animals enters surface waters and rivers in runoff from contaminated pastures. we studied the river tywi in south wales, united kingdom, whose catchment comprises 1,100 km2 containing more than a million dairy and beef cattle and more than 1.3 million sheep. the river tywi is abstracted for the domestic water supply. between august 2002 and april 2003, 48 of 70 (68.8%) twice-weekly river water samples tested positive by is900 pcr. in rive ...200616751517
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