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comparison of indirect fluorescent antibody test (ifat) and slide enzyme linked immunosorbent assay (selisa) for diagnosis of babesia bigemina infection in bovines.an indirect fluorescent antibody test (ifat) and slide enzyme linked immunosorbent assay (selisa) were standardized for the detection of antibodies specific to babesia bigemina in experimentally infected bovine calves and subsequently used for the screening of naturally infected bovine and bubaline sera. in experimentally infected calves positive reactivity was detected in sera at the earliest on day 7 by both the tests. serological studies for detection of b. bigemina specific antibodies in 180 ...200919115088
molecular prevalence of babesia bigemina and trypanosoma evansi in dairy animals from punjab, india, by duplex pcr: a step forward to the detection and management of concurrent latent infections.specific duplex polymerase chain reaction (pcr) was employed on 411 (386 cattle and 25 buffaloes) blood samples of dairy animals from 9 districts of punjab, india, for simultaneous detection of babesia bigemina and trypanosoma evansi. the results were compared and correlated with conventional giemsa stained thin blood smear (gstbs) examination and haematological alterations to know the clinical status and pathogenicity of infections. the bg3/bg4 and tr3/tr4 primers were used in duplex pcr for b. ...201324069605
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