| reverse vaccinology approach identify an echinococcus granulosus tegumental membrane protein enolase as vaccine candidate. | applying reverse vaccinology strategy, we employed a sequence encoding an enolase from taenia asiatica to search its homolog in the expression sequence tag (est) database of echinococcus granulosus and found two est sequences (access number: cn653186 and cn649593) of a clone eg_psgrs_13b09 from e. granulosus protoscolex full-length cdna library, which are responding for the 5' and 3' partial cds of e. granulosus enolase, respectively. primers are designed according to the 5' end and 3'end of the ... | 2010 | 20127115 |