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[allochthonous cases of canine visceral leishmaniasis in paraná, brazil: epidemiological implications].the south region is the only area in brazil that does not present autochthonous cases of visceral leishmaniasis (vl), however, in the state of paraná, dogs and humans have been found showing a vl compatible clinical profile. in view of this problem, the present work aimed at isolating and identifying the parasite and determining the cases autochthony. all animals clinically suspect of vl were clinically evaluated, and had samples of their blood collected for hemoculture (nnn culture medium), ser ...200919772775
the shadows of a ghost: a survey of canine leishmaniasis in presidente prudente and its spatial dispersion in the western region of são paulo state, an emerging focus of visceral leishmaniasis in brazil.visceral leishmaniasis is an emerging zoonosis and its geographic distribution is restricted to tropical and temperate regions. most of the individuals infected in latin america are in brazil. despite the control measures that have been adopted, the disease is spreading throughout new regions of the country. domestic dogs are involved in the transmission cycle and are considered to be the main epidemiologic reservoir of leishmania infantum (syn. l. chagasi). our aim was to determine the prevalen ...201526503368
rural-urban focus of canine visceral leishmaniosis in the far western region of santa catarina state, brazil.the aim of this work was to investigate the occurrence of canine visceral leishmaniosis (cvl) in the far western region of santa catarina state, bordering argentina and parana state, southern brazil, where in recent years, vl has been recorded in both dogs and humans. clinical signs, enzyme-linked immunosorbent assay (elisa), indirect immunofluorescent antibody test (ifat) and polymerase chain reaction (pcr) were used for leishmania investigation. among the 252 dogs surveyed, 41 were positive by ...201425023635
molecular diagnosis of leishmaniosis in the paraná state of southern brazil.the objective of the present study was to establish a polymerase chain reaction (pcr) technique for the diagnosis of cutaneous and mucocutaneous leishmaniosis from autochthonous cases in the state of paraná in southern brazil as well as imported cases. we sought to determine its utility and accuracy compared with smears and present culture methods. to standardize pcr samples, skin and mucosal punch biopsies from human lesions were performed on patients living in different regions of the paraná s ...200818637136
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