| detection of ehrlichia spp. in the blood of wild white-tailed deer in missouri by pcr assay and serologic analysis. | blood samples collected from wild deer in missouri in november of 2000 and 2001 were positive by pcr assays for ehrlichia chaffeensis (50 of 217; 23%), ehrlichia ewingii (44 of 217; 20%), and anaplasma species (214 of 217; 99%). nucleotide sequences of selected amplicons from the assay for anaplasma matched sequences of the white-tailed deer agent. serologic analysis of 112 deer sampled in 2000 showed a very high prevalence of antibodies to e. chaffeensis (97 of 112; 87%) and a low prevalence of ... | 2003 | 12624063 |
| infection rates of amblyomma americanum and dermacentor variabilis by ehrlichia chaffeensis and ehrlichia ewingii in southwest missouri. | both ehrlichia chaffeensis and ehrlichia ewingii are causative agents of human ehrlichiosis. both pathogens are transmitted to humans through the bite of an infected lone star tick (amblyomma americanum). since missouri has a high incidence of human monocytic ehrlichiosis, we investigated the prevalence of e. chaffeensis- and e. ewingii-infected a. americanum and dermacentor variabilis (american dog tick) ticks to help assess the relative risk for humans exposed to these vectors. we used a neste ... | 2002 | 12653298 |
| predominance of ehrlichia ewingii in missouri dogs. | to investigate the species distribution of ehrlichia present in missouri dogs, we tested 78 dogs suspected of having acute ehrlichiosis and 10 healthy dogs. blood from each dog was screened with a broad-range 16s rrna gene pcr assay that detects known pathogenic species of ehrlichia and anaplasma: the species was determined by using species-specific pcr assays and nucleotide sequencing. ehrlichia antibody testing was performed by using an indirect immunofluorescence assay with ehrlichia chaffeen ... | 2003 | 14532192 |
| the pcs20 pcr assay for ehrlichia ruminantium does not cross-react with the novel deer ehrlichial agent found in white-tailed deer in the united states of america. | white-tailed deer are susceptible to heartwater (ehrlichia [cowdria] ruminantium infection) and are likely to suffer high mortality if the disease spreads to the united states. it is vital, therefore, to validate a highly specific and sensitive detection method for e. ruminantium infection that can be reliably used in testing white-tailed deer, which are reservoirs of antigenically or genetically related agents such as ehrlichia chaffeensis, anaplasma (ehrlichia) phagocytophilum (hge agent) and ... | 2004 | 15373331 |
| natural and experimental infection of white-tailed deer (odocoileus virginianus) from the united states with an ehrlichia sp. closely related to ehrlichia ruminantium. | an ehrlichia sp. (panola mountain [pm] ehrlichia sp.) closely related to ehrlichia ruminantium was recently detected in a domestic goat experimentally infested with lone star ticks (lsts, amblyomma americanum) collected from georgia, usa. the infected goat exhibited pyrexia and mild clinical pathologic abnormalities consistent with ehrlichiosis. at least two other ehrlichia species (ehrlichia chaffeensis and ehrlichia ewingii) are maintained in nature by a cycle involving lsts as the primary vec ... | 2008 | 18436670 |
| evaluation of peptide- and recombinant protein-based assays for detection of anti-ehrlichia ewingii antibodies in experimentally and naturally infected dogs. [corrected] | to evaluate microtiter-plate format elisas constructed by use of different diagnostic targets derived from the ehrlichia ewingii p28 outer membrane protein for detection of e ewingii antibodies in experimentally and naturally infected dogs. | 2010 | 20919906 |
| detection of borrelia, ehrlichia, and rickettsia spp. in ticks in northeast missouri. | we evaluated amblyomma americanum (lone star tick) and dermacentor variabilis (american dog tick) in northeast missouri for the presence of borrelia, ehrlichia, and rickettsia bacteria. we collected actively questing ticks from four sites within adair county, missouri. a total of 15,162 ticks were collected, of which 13,980 were grouped in 308 pools (lone star ticks, 288 pools; american dog ticks, 20 pools) and tested for presence/absence of bacteria using polymerase chain reaction. infection ra ... | 2016 | 27133163 |