| molecular epidemiology of plasmodium vivax in the state of amazonas, brazil. | over the past 2 decades, the amazon region of brazil has experienced reemergence of plasmodium vivax malaria, with reported occurrence of severe disease. the frequency and manifestations of this severe disease are unlike previous clinical experience. the hypothesis has been raised that the occurrence of severe disease may relate to the emergence of a variant form of the parasite. to test this hypothesis, we conducted a retrospective cohort study of p. vivax strains in the state of amazonas. we d ... | 2007 | 17397788 |
| polymorphism at the merozoite surface protein-3alpha locus of plasmodium vivax: global and local diversity. | allelic diversity at the plasmodium vivax merozoite surface protein-3alpha (pvmsp-3alpha) locus was investigated using a combined polymerase chain reaction/restriction fragment length polymorphism (pcr/rflp) protocol. symptomatic patient isolates from global geographic origins showed a high level of polymorphism at the nucleotide level. these samples were used to validate the sensitivity, specificity, and reproducibility of the pcr/rflp method. it was then used to investigate pvmsp3alpha diversi ... | 1999 | 10548283 |
| antibody response of naturally infected individuals to recombinant plasmodium vivax apical membrane antigen-1. | in the present study, we evaluate the naturally acquired antibody response to the plasmodium vivax apical membrane antigen 1 (pvama-1), a leading vaccine candidate against malaria. the gene encoding the pvama-1 ectodomain region (amino acids 43-487) was cloned by pcr using genomic dna from a brazilian individual with patent p. vivax infection. the predicted amino acid sequence displayed a high degree of identity (97.3%) with a previously published sequence from the p. vivax salvador strain. a re ... | 2004 | 15710439 |