Publications

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electrophoretic variants of enzymes in isolates of plasmodium falciparum, p. malariae and p. vivax from thailand.a new electrophoretic variant of glucose phosphate isomerase (gpi), which we now denote gpi-3, has been found in isolates of plasmodium falciparum from 6 patients, all of whom acquired the infection in the same region (in or near prachinburi province) of thailand. in other regions, from which 453 isolates have been tested, only gpi-1 and/or gpi-2 have been found. two isolates of p. malariae from patients at kanchanaburi showed a band of gpi activity on cellulose acetate gels at a cathodal positi ...19892694498
investigation of malaria sporozoites by immunoradiometric assay.two-site immunoradiometric assay (irma) (zavala et al., 1982) using monoclonal antibodies to p. falciparum and p. vivax was applied to detect sporozoites in laboratory-maintained an. dirus and also mosquitoes collected from endemic areas of malaria in thailand. study in p. falciparum infected mosquitoes revealed that the circumsporozoite (cs) antigen was first found in the abdominal portion on day 10 post-infection, while it could be observed in the salivary glands from day 15 onwards. the head- ...19883043705
comparison between microscopic examination, elisa and quantitative buffy coat analysis in the diagnosis of falciparum malaria in an endemic population.monoclonal antibody-based elisa and qbc (quantitative buffy coat analysis) were tested in two endemic areas with low and high incidence of malaria in kanchanaburi province, west thailand with annual parasite incidence in 1992 of 119 and 5 per 1,000 population, respectively. the numbers of individuals positive by thick blood film examination (tbf) for p. falciparum with or without p. vivax, and p. vivax only were 82 and 69, respectively. the detection limit of elisa was 10 parasites/10(6) red blo ...19958525418
prevention of sporogony of plasmodium vivax in anopheles dirus mosquitoes by transmission-blocking antimalarials.the sporontocidal activity of four dihydroacridine-diones (wr-233602, wr-243251, wr-250547, and wr-250548) and three fluoroquinolones (wr-279135, wr-279298, and wr-279288) was determined against naturally circulating isolates of plasmodium vivax. laboratory-reared anopheles dirus mosquitoes were infected with p. vivax by feeding them on gametocytemic volunteers reporting to local malaria clinics in kanchanaburi and tak provinces, thailand. four days after the infectious feed, mosquitoes were re- ...200111561707
field evaluation of the ict malaria pf/pv immunochromatographic test for the detection of asymptomatic malaria in a plasmodium falciparum/vivax endemic area in thailand.rapid antigen assays provide an effective tool for the detection of malaria in symptomatic patients. however, the efficacy of these devices for detecting asymptomatic malaria, where parasite levels are normally significantly lower than in symptomatic patients, is less well established. we evaluated the efficacy of a new combined plasmodium falciparum-plasmodim vivax immunochromatographic test (ict malaria pf/pv) in a cross-sectional malaria survey of the village of ban kong mong tha, kanchanabur ...200212164291
detection of putative antimalarial-resistant plasmodium vivax in anopheles vectors at thailand-cambodia and thailand-myanmar borders.monitoring of multidrug-resistant (mdr)falciparum and vivax malaria has recently been included in the global plan for artemisinin resistance containment (gparc) of the greater mekong sub-region, particularly at the thailand-cambodia and thailand-myanmar borders. in parallel to gparc, monitoring mdr malaria parasites in anopheline vectors is an ideal augment to entomological surveillance. employing plasmodium- and species-specific nested pcr techniques, only p. vivax was detected in 3/109 salivar ...201627244954
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