| different strategies for molecular differentiation of mycobacterium bovis strains isolated in sardinia, italy. | different genetic markers were used to analyze 22 mycobacterium bovis strains isolated from cattle in sardinia and one human isolate. is6110 dna fingerprinting differentiated the strains into six patterns, whereas with enterobacterial repetitive consensus sequence primers produced seven clusters. pcr ribotyping followed by digestion with haeiii and pvuii produced five and seven patterns, respectively. pcr with the (gtg)5 oligonucleotide primer showed the best discriminatory power, generating eig ... | 1999 | 10103282 |
| molecular basis of rifampin and isoniazid resistance in mycobacterium bovis strains isolated in sardinia, italy. | fourteen of 22 (68%) mycobacterium bovis strains isolated from cattle in sardinia were found to be resistant to rifampin and isoniazid. analysis of the rpob and the katg, oxyr-ahpc, and inha gene regions of these strains was performed in order to investigate the molecular basis of rifampin and isoniazid resistance, respectively. the most frequent mutation, encountered in 6 of 10 strains (60%), was in the rpob gene; it occurred, at codon position 521 and resulted in leucine changed to proline. th ... | 2001 | 11353606 |