molecular characterization of mycobacterium bovis isolates from ethiopian cattle.bovine tuberculosis (btb) is a widespread and endemic disease of cattle in ethiopia. information relating to genotypic characteristics of mycobacterium bovis strains affecting the cattle population in ethiopia is limited. we carried out molecular characterization of m. bovis strains isolated from btb infected cattle using the spoligotyping technique. the relationship between distribution of spoligotypes and recorded variables was also investigated. a new approach that can numerically reflect the ...201020507576
status of bovine tuberculosis in addis ababa dairy farms.the study was conducted to determine the status of bovine tuberculosis in addis ababa, ethiopia, by a comparative intradermal tuberculin test of 1,869 animals in 106 farms. epidemiological information was also collected, taking into account factors chosen for their epidemiological significance and local livestock husbandry characteristics. in addition, milk samples were collected from tuberculin reactors for mycobacterial isolation and characterisation. chi-square statistic, simple regression an ...200819284060
effect of skin testing and segregation on the prevalence of bovine tuberculosis, and molecular typing of mycobacterium bovis, in 2002, the prevalence of bovine tuberculosis (tb) among 500 cattle on holeta farm, near addis ababa, ethiopia, was 48 per cent, and the farm was divided into positive and negative herds. after three consecutive rounds of skin testing and segregation of skin test-positive and -negative animals, the prevalence of bovine tb was reduced from 14 per cent to 1 per cent in the negative herd in a year. spoligotyping of 41 isolates from 17 cows gave an identical and unique spoligotype pattern, which ca ...200718065813
diagnostic efficiency of abattoir meat inspection service in ethiopia to detect carcasses infected with mycobacterium bovis: implications for public health.bovine tuberculosis (btb) is a widespread and endemic disease of cattle in ethiopia posing a significant threat to public health. regular surveillance by skin test, bacteriology and molecular methods is not feasible due to lack of resource. thus, routine abattoir (ra) inspection will continue to play a key role for national surveillance. we evaluated efficiency of ra inspection for diagnosis of mycobacterium bovis infection and discussed its public health implications in light of a high risk of ...201020691081
prevalence of bovine tuberculosis in ethiopian slaughter cattle based on post-mortem examination.a study aimed at describing the magnitude and distribution of gross lesions compatible with bovine tuberculosis (btb) in ethiiopian slaughter cattle in five abattoirs (four municipal and one export) located in various cattle husbandry systems in ethiopia was carried out from july 2006 to january 2007 using detailed meat inspection procedure. five representative abattoirs (four municipal and one export) located in distinct livestock management systems were selected. a total of 3322 cattle; 2876 ( ...200919058024
evaluation of abattoir inspection for the diagnosis of mycobacterium bovis infection in cattle at addis ababa abattoir.detailed postmortem examinations were conducted to evaluate the efficiency of meat inspection procedures and to determine the distribution of lesions in mycobacterium bovis-infected cattle. the study involved routine inspection at slaughter, collection of tissues for detailed examination in the laboratory, and bacteriological examination to identify m. bovis. additionally, a 10-year (1992--2001) meat inspection record was analysed to determine tuberculosis trends in the past decade. chi2-test an ...200415560514
prevalence study on bovine tuberculosis and molecular characterization of its causative agents in cattle slaughtered at addis ababa municipal abattoir, central ethiopia.a cross-sectional study was conducted on 500 cattle slaughtered at addis ababa abattoir to determine the prevalence of bovine tuberculosis (btb) and characterize its causative agents. postmortem examination, mycobacteriological culturing, region of difference-4 (rd4)-based pcr and spoligotyping were applied. the prevalence of btb was 5 % on the basis of postmortem inspection alone but 1.2 % based on molecular confirmation. factors including age, sex, and breed showed statistically significant as ...201323065393
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