| development of a supersensitive polymerase chain reaction method for human t lymphotropic virus type ii (htlv-ii) and detection of htlv-ii proviral dna from blood donors in japan. | a supersensitive polymerase chain reaction procedure was developed to detect human t-lymphotropic virus type ii (htlv-ii) proviral genome. six primer pairs covering the various regions of htlv-ii were compared and selected on the basis of specificity and sensitivity. among them, one primer pair of the pol region of htlv-ii (ii pol) was able to amplify and detect even 0.1 fg of the cloned plasmid htlv-ii dna (seven copies) by regular ethidium bromide staining on polyacrylamide gel. by using this ... | 1992 | 1589961 |