Publications

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association of bola drb3 and dqa1 alleles with susceptibility to neospora caninum and reproductive outcome in quebec holstein cattle.the bola drb3 and dqa1 genes are part of the major histocompatibility complex (mhc) class ii in cattle. these genes are highly polymorphic and have been associated with resistance to several diseases, such as mastitis, bovine leukemia virus (blv) and dermatophilis. sequenced based typing of these genes has been carried out extensively from blood samples; however it is often impractical or expensive to obtain such samples. repositories of well-characterized serum from cattle are readily available ...200919646818
production effects of pathogens causing bovine leukosis, bovine viral diarrhea, paratuberculosis, and neosporosis.the primary purpose of this research was to determine associations among seropositivity for bovine leukemia virus (blv), bovine viral diarrhea virus (bvdv), mycobacterium avium ssp. paratuberculosis (map), and neospora caninum (nc) and each of 3 outcome variables (305-d milk, fat, and protein production) in canadian dairy cattle. serum samples from up to 30 randomly selected cows from 342 herds on monthly milk testing were tested for antibodies against blv (idexx elisa; idexx corporation, westbr ...200717235141
importance of primer selection in the application of pcr technology to the diagnosis of bovine leukemia virus.the polymerase chain reaction (pcr) was used to detect bovine leukemia virus in bovine blood samples. when applied to leucocytes extracted from the blood samples, the standard method of dna extraction gave good correlation with agar gel immunodiffusion, but a method in which 5 microliters of blood was the starting material was unreliable. selection of the primers was important, and differences in results were observed when the pcr method was applied to blood samples from different geographic are ...19947948198
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