epidemiology of hydatid disease in sardinia: a study of fertility of cysts in sheep.hydatidosis, caused by echinococcus granulosus, is a cyclozoonotic disease of economic significance in sardinia. the life-cycle involves stray and sheep dogs as definitive hosts and sheep, pigs, goats and cattle as intermediate hosts. the most important intermediate host is sheep, due to home slaughtering with ready access of the viscera to dogs. this survey was undertaken in 1987 to ascertain the epidemiological significance of sheep in maintaining the life-cycle. a total of 700 (91.3%) of 767 ...19902230030
chronobiology of oestrus ovis (diptera: oestridae) in sardinia, italy: guidelines to chemoprophylaxis.oestrus ovis (linné 1761) larvae are obligatory parasites of the nasal and sinus cavities of sheep and goats. infestation is prevalent in hot and dry regions, such as mediterranean countries. the current work was developed to establish the chronobiology of o. ovis in sardinia, to determine the most suitable time for chemoprophylaxis. a survey was carried out during 1998, and sheep heads were collected monthly from local flocks. a total of 443 heads was examined, and the prevalence of oestrosis w ...200212144298
haplotype affinities resolve a major component of goat (capra hircus) mtdna d-loop diversity and reveal specific features of the sardinian stock.goat mtdna haplogroup a is a poorly resolved lineage absorbing most of the overall diversity and is found in locations as distant as eastern asia and southern africa. its phylogenetic dissection would cast light on an important portion of the spread of goat breeding. the aims of this work were 1) to provide an operational definition of meaningful mtdna units within haplogroup a, 2) to investigate the mechanisms underlying the maintenance of diversity by considering the modes of selection operate ...201222363488
identification of single nucleotide polymorphisms in the nicastrese goat and sardinia sheep mannose-binding lectin.this study was undertaken to detect polymorphisms in the goat and sheep mannose-binding lectin encoding gene (mbl2) and to explore allelic variability of this gene in these two species. the analysis and comparison of the sequences obtained from sheep showed 13 polymorphic sites, six in the promoter and seven in exon 1, four of which were of the missense type. in the goats, 12 polymorphic sites were detected, five intronic, five in the promoter, and one exonic. the exon site was responsible for a ...201121948269
detection of pathogens in ovine and caprine abortion samples from sardinia, italy, by pcr.during 2003-2005, 399 abortion samples (315 fetuses and 84 placentae) were collected from 107 ovine and caprine farms in northern sardinia. tissues from aborted fetuses and placentae were examined by pcr assay to detect dna from coxiella burnetii, chlamydophila abortus, salmonella enterica serovar abortusovis, toxoplasma gondii, and neospora caninum. the dna from at least 1 of these 5 infectious agents was amplified in 41% of ovine fetuses, while only 17% of the caprine fetuses yielded a positiv ...200717459840
esr dating of an ancient goat tooth from nuoro, sardinia, italy.the dating of fossil teeth of an ancient goat (nesogoral melonii) using the electron spin resonance (esr) technique is reported. this animal was found in the fossiliferous site at orosei (nuoro, sardinia, italy) and was endemic in the region. molar teeth were cleaned and enamel was completely removed from dentine. enamel was irradiated with a 60co gamma source and measured with an esr spectrometer (x-band) to obtain the signal vs. dose curve and fitted with an exponential function. the archeolog ...200616597696
small ruminant lentivirus genotype e is widespread in sarda goat.the highly divergent srlv genotype e has recently been characterized in italy as a low pathogenic caprine lentivirus in the roccaverano breed. the availability of a genotype specific diagnostic test based on a comparative assay, using a combination of genotype specific recombinant antigens allows a wide serosurvey in other goat populations. the island of sardinia still has the highest small ruminant population of any italian region and crossbreeding has been limited to goats, mainly with the mal ...201020060658
role of chlamydophila abortus in ovine and caprine abortion in sardinia, italy.between 1999-2003, 14321 sera and 646 abortion samples (498 foetuses and 148 placentae) were analysed from 807 sheep and goat farms distributed all over the island of sardinia. after notification of abortion in a flock, sera collected at random from adult animals were examined to detect antibodies specific to chlamydophila (c.) abortus by elisa, whereas foetuses and placenta were analysed by pcr assay. specific igg antibodies were detected in 611 (4.8%) sheep and 106 (5.8%) goats. from a total o ...200515943071
occurrence, distribution, and role in abortion of coxiella burnetii in sheep and goats in sardinia, italy.between 1999 and 2002, 9349 sera and 517 aborted samples (422 foetuses and 95 placenta) were analysed from 675 sheep and 82 goat farms distributed all over the island of sardinia. after abortion notification, sera collected at random from adult animals were examined to detect antibodies specific to coxiella burnetii by elisa, whereas foetuses and placenta were analysed by pcr assay. specific igg antibodies were detected in 255 (38%) sheep farms and in 39 (47%) goat herds whereas 40 ovine (10%) a ...200415066733
survey of ovine and caprine toxoplasmosis by ifat and pcr assays in sardinia, italy.during the period 1999-2002, we have analyzed 9639 serum samples and 815 aborted samples (670 fetuses and 145 placenta) from 964 ovine and caprine farms distributed over all sardinia island. after abortion notification, sera collected at random from adult animals were examined to detect simultaneously igg and igm antibodies specific to toxoplasma gondii by indirect immunofluorescence assay, whereas fetuses and placenta were analyzed by a single tube nested pcr assay. specific igg antibodies were ...200314597274
assessing genetic diversity in italian goat populations using aflp markers.amplified fragment length polymorphism (aflp) markers were used to investigate the genetic variation in a sample of seven goat (capra hircus) populations. a total of 210 individuals (30 per population) were analysed using seven selected aflp primer combinations that produced 219 clear polymorphisms. four autochthonous goat breeds (bionda dell'adamello, frisa, orobica and verzaschese), two primary populations, one from the lombardy alps (val di livo) and the other from sardinia island (sarda) and ...200111683715
ticks infesting domestic animals in italy: current acarological studies carried out in sardinia and basilicata regions.the note reviews published and unpublished data on acaralogical studies carried out by different parasitologists' groups in sardinia and basilicata regions. in sardinia 72 collecting sites were investigated and a total of 4,086 ticks were collected from hosts (cattle, sheep, goats and pigs) and from soil. so far 2,413 ticks belonging to six genera have been identified: rhipicephalus (67.2%), haemaphysalis (24.1%), hyalomma (2.7%), boophilus (0.7%), dermacentor (4.9%), ixodes (0.3%). host-associa ...199911071541
characterization of yeast population and molecular fingerprinting of candida zeylanoides isolated from goat's milk collected in sardinia.the occurrence of yeast microflora in raw goat's milk collected from 62 dairy farms located in different areas of sardinia was evaluated. candida zeylanoides was the most frequently occurring species followed by different basidiomycetous species. in the strains isolated some biochemical characteristics of technological interest were investigated and a predominance of lipolytic yeast species was found. we employed a simple method of dna extraction that in a minimal time and with low-cost provided ...201019879008
cystic echinococcosis in italy from the 1950s to italy the epidemiological pattern of cistic echinococcosis (ce) is incomplete and the information for most regions is out of date, contradictory, and almost exclusively limited to the intermediate hosts. the disease is found most frequently in particular social and economic conditions: widespread use of extensive or semi-extensive sheep farming, illegal slaughtering, and high numbers of sheepdogs and other types of dogs. the highest incidence in sheep is found in sardinia (70.6-92.8%), sicily ...200416044697
analysis of pharmaceutically relevant taxoids in wild yew trees from sardinia.needles from a series of wild yews (taxus baccata l.) from sardinia were investigated for their contents of 10-deacetyl baccatin iii (dab-iii, 1), paclitaxel (taxol) (2) and taxine (3). despite a common geographical origin, ample variation of the taxoid profile was discovered, and several samples were surprisingly devoid of all terpenoid markers above. this finding is unprecedented within the european yew, while the general lack of taxine might rationalize the observation that most plants invest ...200312628392
[further inquiries on paramphistomosis (paramphistomum cervi) in sardinia]. 196314123058
specific igg antibody responses in oestrus ovis l. (diptera: oestridae) infected sheep: associations with intensity of infection and larval development.larvae of oestrus ovis (diptera: oestridae) are ubiquitous parasites of nasal and sinusal cavities of sheep and goats. according to the chronobiology of o. ovis infections in sardinia and the seasonal pattern of the igg response, the optimal period to investigate the relationships between o. ovis larval populations and intensity of local and systemic igg antibody responses was mid-july in the summer season. sarda x lacaune ewes (n=186), divided into three ram-families were used in the study. sys ...200818584967
[epidemiology and social/health implications of hydatidosis in sardinia].all surgically treated cases of hydatidosis registered in sardinia from 1969 to 1984 have been analysed. the 16-year period clearly shows that unilocular hydatid disease caused by larvae of tapeworm, echinococcus granulosus, is endemic in sardinia. in this region there are foci of human infection notably in the sheep rearing areas, of nuoro and sassari. the incidence of human hydatidosis shows a marked regional variation varying during the last survey (1982-1984) between 16.9/1000 in the provinc ...19892491290
sheep oestrosis (oestrus ovis linné 1761, diptera: oestridae) in sardinia, italy.oestrosis, the nasal myiasis of sheep and goats, is caused by the larvae of oestrus ovis l. 1758 (diptera, oestridae) that develop from the first to the third stage larva in the nasal cavities and frontal sinuses of affected animals. the authors report the results of an epidemiological study of oestrosis of sheep in sardinia, italy. heads of 6-month to 10-year-old sardinian sheep (n=566) from 124 free-ranging flocks were examined for the presence and location o. ovis larvae from december 1996 to ...200111705659
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