| [the first report of an epidemic of human metapneumovirus infection in japan: clinical and epidemiological study]. | human metapneumovirus (hmpv) was newly discovered as a pathogen in 2001 and is thought to be associated with respiratory disease. to elucidate the prevalence and clinical significance of hmpv among children, we investigated the positive cases of hmpv-rna by reverse transcription-polymerase chain reaction (rt-pcr) in their nasopharyngeal specimens collected from january to august 2003 in hiroshima prefecture, japan. our prospective study revealed 77 hmpv-positive cases among 377 children with acu ... | 2004 | 15103904 |
| outbreak of human metapneumovirus detected by use of the vero e6 cell line in isolates collected in yamagata, japan, in 2004 and 2005. | a number of epidemiological studies have shown human metapneumovirus (hmpv) to be one of the most important viral agents associated with acute respiratory infections in humans. however, due to the difficulty in growing the virus, all epidemiological studies of hmpv infection have been performed on the basis of the molecular method. thus, the development of a cell line suitable for the isolation of hmpv from clinical specimens is a crucial step for further research. using the vero e6 cell line, w ... | 2007 | 17460056 |
| clinical impact of human metapneumovirus genotypes and genotype-specific seroprevalence in yamagata, japan. | the clinical impact of human metapneumovirus (hmpv) genotypes and the relation between the hmpv genotype in circulation and genotype-specific seroprevalence are yet to be clarified. we determined the genotypes of 93 hmpv strains that were isolated between 2004 and 2006 in yamagata, japan, and identified 35 genotype a2, 14 genotype b1, and 44 genotype b2 isolates. children infected with genotype a2 hmpv were significantly older than those infected with genotype b1 hmpv. diagnosis of laryngitis wa ... | 2008 | 18428137 |
| analysis of monthly isolation of respiratory viruses from children by cell culture using a microplate method: a two-year study from 2004 to 2005 in yamagata, japan. | although well over 200 viral agents have been implicated in acute respiratory infections (aris) among children, no system able to detect such a wide range of viruses has been established. between january 2004 and december 2005, a modified microplate method, including hef, hep-2, vero e6, mdck, rd-18s, and gmk cell lines (hhve6mrg plate), was adopted to isolate viruses. a total of 1,551 viruses were isolated, representing both outbreaks and sporadic cases, from 4,107 nasopharyngeal specimens, at ... | 2008 | 18503168 |
| human metapneumovirus infection in febrile children with lower respiratory diseases in primary care settings in hiroshima, japan. | human metapneumovirus (hmpv) has been shown to be a leading cause of viral lower respiratory tract infections in children. nevertheless, few reports regarding hmpv infections over consecutive years in children in primary care settings are available. we carried out virologic and clinical studies to determine the role of hmpv in febrile lower respiratory infections in children at a primary care clinic over 3 years and 5 months. nasopharyngeal aspirates obtained from children with acute respiratory ... | 2008 | 19050367 |
| endemicity of human metapneumovirus subgenogroups a2 and b2 in yamagata, japan, between 2004 and 2009. | to clarify a longitudinal epidemiology,we isolated 280 hmpv strains from patients with acute respiratory infections in yamagata, japan, between 2004 and 2009.we observed that the high season for hmpv was from winter to spring (between january and may) and the low season was in the fall (around september and october). a further molecular analysis revealed that subgenogroup a2 (a2) strains were the most commonly isolated (151/280; 53.9%), followed by b2 (108/280; 38.6%) and b1 (19/280; 6.8%). our ... | 2010 | 21140598 |