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a bowman-birk trypsin inhibitor with antiproliferative activity from hokkaido large black soybeans.a trypsin inhibitor, with an n-terminal sequence highly homologous to those of 8-kda bowman-birk trypsin inhibitors, was isolated from the seeds of hokkaido large black soybeans. the trypsin inhibitor was unadsorbed on sp-sepharose but adsorbed on deae-cellulose and mono q. it inhibited proliferation in breast cancer (mcf-7) cells and hepatoma (hep g2) cells with an ic50 of 35 and 140 microm, respectively. the trypsin inhibitory activity of the inhibitor was completely preserved after exposure t ...200817880027
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