| molecular characterization of gentamicin-resistant enterococci in the united states: evidence of spread from animals to humans through food. | we evaluated the molecular mechanism for resistance of 360 enterococci for which the gentamicin mics were >/=128 micro g/ml. the aac(6')-ie-aph(2")-ia, aph(2")-ic, and aph(2")-id genes were identified by pcr in isolates from animals, food, and humans. the aph(2")-ib gene was not identified in any of the isolates. two enterococcus faecalis isolates (mics > 1,024 micro g/ml) from animals failed to generate a pcr product for any of the genes tested and likely contain a new unidentified aminoglycosi ... | 2003 | 12624037 |
| induction of the methionine-activating enzyme in saccharomyces cerevisiae. | pigg, c. joanne (oregon state university, corvallis), w. a. sorsoli, and l. w. parks. induction of the methionine-activating enzyme in saccharomyces cerevisiae. j. bacteriol. 87:920-923. 1964.-the methionine-activating enzyme was induced in yeast in the presence of methionine, resulting in an accumulation of s-adenosylmethionine. formation of the sulfonium derivative prevented the accumulation of high levels of free methionine in induced cells. the physiological significance of this phenomenon i ... | 1964 | 14137631 |
| geographical differences in bacteria detected in endodontic infections using polymerase chain reaction. | the polymerase chain reaction (pcr) is an innovative nucleic acid-based assay that has the highest sensitivity of any microbiological technique for the detection of bacteria. the purpose of this study was to use pcr to detect the presence of specific species of bacteria in samples collected from two geographical locations. microbial samples from abscesses of endodontic origin were collected from patients in portland, oregon, and rio de janeiro, brazil. pcrs with species-specific oligonucleotide ... | 2004 | 15055430 |
| detection of blastocystis from stool samples using real-time pcr. | we developed a real-time lc pcr assay to detect a 152 bp sequence in an uncharacterized region of the blastocystis genome. the described assay detected 11 of 11 atcc strains of blastocystis from subtypes 1, 3, and 4. three of three stool samples from oregon and california military personnel that were negative for blastocystis by an ova and parasite test as well as a conventional pcr assay were positive for blastocystis using our real-time lc pcr assay. diagnosis of blastocystis infections using ... | 2008 | 18488250 |