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molecular detection of virulence factors and antibiotic resistance pattern in clinical enterococcus faecalis strains in sardinia.in this study, the antibiotic resistance pattern and the presence of genes encoding several virulence factors in 91 enterococcus faecalis strains isolated from different human clinical sources in sardinia were investigated. genotypic determination of virulence genes (gele, esp, agg, ace, cyla,b,m,l(l),l(s), efaa, fsrb) was carried out by pcr. the production of gelatinase and haemolytic activity were also determined. antimicrobial susceptibility tests were performed by an automated microdilution ...201020853674
chemical compositions of the essential oils of aerial parts of chamaemelum mixtum (l.) alloni.the chemical compositions of the aerial parts essential oils of chamaemelum mixtum (l.) alloni from corsica and sardinia were investigated employing gas chromatography and gas chromatography/mass spectrometry. the structure of (z)-heptadeca-9,16-dien-7-one, a natural compound not previously described, was elucidated by gas chromatography/mass spectrometry (electron impact and chemical ionization) and one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy. the variation in ch ...201222225420
outbreak of mastitis in sheep caused by multi-drug resistant enterococcus faecalis in sardinia, italy.an outbreak of infective mastitis due to enterococcus faecalis occurred in an intensive sheep farm in north sardinia (italy). e. faecalis, which is only rarely isolated from sheep milk, was unexpectedly found in 22·3% of positive samples at microbiological examination. forty-five out of the 48 e. faecalis isolates showed the same multi-drug resistance pattern (cloxacillin, streptomycin, kanamycin, clindamycin, oxytetracycline). e. faecalis isolates were analysed by pulsed-field gel electrophores ...201322595402
incidence of virulence determinants in clinical enterococcus faecium and enterococcus faecalis isolates collected in sardinia (italy).enterococci are widely distributed in the environment; within the human body, they are normal commensals of the oral cavity, gastrointestinal tract and vagina. in recent years, enterococci have become one of the most frequent causes of acquired nosocomial infections worldwide. the molecular mechanism of virulence of these bacteria is still not completely understood. the aims of this work were to characterize phenotypically 47 isolates of enterococcus faecalis and enterococcus faecium collected i ...200312748268
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