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[prevalence of pertussis in fukuoka: incidence and mics of antibiotics for bordetella pertussis isolate].for bacteriological examinations of whooping cough patients, nasopharyngeal specimens were directly inoculated on the cyclodextrin solid medium (csm) supplemented with 5 micrograms of cephalexin (cex) per ml. the inoculated plates were cultured in an incubator at 35 degrees c for 3 to 7 days. during the period from 1990 to 1993, b. pertussis (43 strains) and b. parapertussis (1 strain) were isolated from 145 whooping cough patients and 34 relatives. b. pertussis were isolated sufficiently in jun ...19957594779
chemical studies on cellular components of bordetella pertussis. iii. isolation of highly potent toxin from bordetella pertussis.onoue, kaoru (kyushu university, fukuoka, japan), masayasu kitagawa, and yuichi yamamura. chemical studies on cellular components of bordetella pertussis. iii. isolation of highly potent toxin from bordetella pertussis. j. bacteriol. 86:648-655. 1963.-the thermolabile toxin of bordetella pertussis was extracted at alkaline ph with 0.15 m saline from the disrupted cells. the toxin was purified successively by calcium phosphate gel treatment, ammonium sulfate fractionation, precipitation with pota ...196314066457
seroepidemiologic study on pertussis, diphtheria, and tetanus in the fukuoka area of southern japan: seroprevalence among persons 0-80 years old and vaccination program.in japan, mass vaccination for diphtheria, pertussis, and/or tetanus has been mandated by the vaccination law since 1948. in order to evaluate the efficacy of this vaccination policy, we conducted seroepidemiological studies on pertussis, diphtheria, and tetanus among individuals aged 0 - 80 years. the pertussis toxin seropositive rates of the vaccine-eligible groups and vaccine-ineligible groups were 55.0 and 57.9%, respectively. the seropositive rate of each group for diphtheria antitoxin was ...200415118214
chemical studies on cellular components of bordetella pertussis. i. purification and properties of agglutinogen.onoue, kaoru, (kyushu university, fukuoka, japan), masayasu kitagawa, and yuichi yamamura. chemical studies on cellular components of bordetella pertussis. i. purification and properties of agglutinogen. j. bacteriol. 82:648-656. 1961.-a method is described for the preparation of the agglutinogen of hemophilus pertussis in phase i by methanol precipitation and ion-exchange chromatography. high homogeneity of this agglutinogen was evidenced by chromatography, but a trace of impurity was detected ...196114482070
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