| experimental and serologic observations on avian pneumovirus (apv/turkey/colorado/97) infection in turkeys. | an avian pneumovirus (apv) was isolated from commercial turkeys in colorado (apv/colorado) showing clinical signs of a respiratory disease. the results of virus neutralization and indirect fluorescent antibody tests showed that the apv/colorado was partially related to apv subgroup a but was unrelated to apv subgroup b. turkeys experimentally inoculated with the apv/colorado were observed for signs, lesions, seroconversion, and virus shedding. thirty-six 7-wk-old turkeys were distributed into th ... | 2000 | 10737640 |
| specific detection of avian pneumovirus (apv) us isolates by rt-pcr. | this report details the development of an rt-pcr assay for the specific detection of us isolates of avian pneumovirus (apv). of the several primer pairs tested, two sets of primers derived from the matrix gene of apv were able to specifically detect the viral rna of apv. the nucleotide sequence comparison of the pcr products of apv isolates from minnesota suggested that these viruses were closely related to the colorado strain of apv, but were distinct from subtypes a and b european isolates of ... | 2000 | 10948996 |
| the sensitivity and specificity of a reverse transcription-polymerase chain reaction assay for the avian pneumovirus (colorado strain). | a reverse transcription-polymerase chain reaction (rt-pcr) assay for the detection of avian pneumovirus (apv), colorado strain (us/co), was evaluated for sensitivity and specificity. the single-tube rt-pcr assay utilized primers developed from the matrix (m) gene sequence of the us/co apv. the rt-pcr amplified the us/co apv but did not amplify other pneumoviruses, including the avian pneumoviruses subgroups a and b. the rt-pcr was capable of detecting between 10(0.25) mean tissue culture infecti ... | 2000 | 11007019 |
| molecular epidemiology of subgroup c avian pneumoviruses isolated in the united states and comparison with subgroup a and b viruses. | the avian pneumovirus (apv) outbreak in the united states is concentrated in the north-central region, particularly in minnesota, where more outbreaks in commercial turkeys occur in the spring (april to may) and autumn (october to december). comparison of the nucleotide and amino acid sequences of nucleoprotein (n), phosphoprotein (p), matrix (m), fusion (f), and second matrix (m2) genes of 15 u.s. apv strains isolated between 1996 and 1999 revealed between 89 and 94% nucleotide sequence identit ... | 2002 | 11980943 |
| nucleotide and predicted amino acid sequence-based analysis of the avian metapneumovirus type c cell attachment glycoprotein gene: phylogenetic analysis and molecular epidemiology of u.s. pneumoviruses. | a serologically distinct avian metapneumovirus (ampv) was isolated in the united states after an outbreak of turkey rhinotracheitis (trt) in february 1997. the newly recognized u.s. virus was subsequently demonstrated to be genetically distinct from european subtypes and was designated ampv serotype c (ampv/c). we have determined the nucleotide sequence of the gene encoding the cell attachment glycoprotein (g) of ampv/c (colorado strain and three minnesota isolates) and predicted amino acid sequ ... | 2003 | 12682171 |
| deduced amino acid sequence of the small hydrophobic protein of us avian pneumovirus has greater identity with that of human metapneumovirus than those of non-us avian pneumoviruses. | we report here the nucleotide and deduced amino acid (aa) sequences of the small hydrophobic (sh) gene of the avian pneumovirus strain colorado (apv/co). the sh gene of apv/co is 628 nucleotides in length from gene-start to gene-end. the longest orf of the sh gene encoded a protein of 177 aas in length. comparison of the deduced aa sequence of the sh protein of apv/co with the corresponding published sequences of other members of genera metapneumovirus showed 28% identity with the newly discover ... | 2003 | 12727346 |
| seroprevalence of avian pneumovirus in minnesota turkeys. | avian pneumovirus (apv) causes respiratory tract infection in turkeys and was first seen in the united states in colorado in late 1996. in early 1997, the disease was recognized in minnesota and caused estimated losses of up to 15 million dollars per year. this virus has not been reported in the other turkey producing states. we here report the seroprevalence of apv in minnesota from august 1998 to july 2002. the average rate of seroprevalence has been 36.3% (range = 14.2%-64.8%). a seasonal bia ... | 2003 | 14562899 |