prevalence and pathogenicity of entamoeba histolytica in three different regions of pernambuco, northeast brazil.parasitological examinations were carried out on 663 individuals of three different cities of pernambuco state, northeastern brazil: recife, palmares and bodocó. the population from a drought area of pernambuco state, bodocó, was investigated for amoebiasis and compared with recife, metropolitan city (about 1.3 million of inhabitants) and another inland community, palmares, located inside of the sugar-cane plantation region of the state. no evidence of invasive strains of e. histolytica were fou ...19947569626
[occurrence of entamoeba histolytica/entamoeba dispar in ambulatory patients of recife, pe].the objective this study was to determine the occurrence of the species entamoeba histolytica/entamoeba díspar in clinical samples of ambulatory patients in pernambuco. a specific assay for entamoeba histolytica was used in this study, which identified no positive fecal samples among the 213 patients. these results confirm that e. dispar is the dominant species in pernambuco state.200617119758
determination of the prevalence of entamoeba histolytica and e. dispar in the pernambuco state of northeastern brazil by a polymerase chain reaction.previous studies using methods varying from traditional serologic tests to molecular biology techniques have shown that in northeastern brazil, entamoeba dispar was more prevalent than e. histolytica. in this study, the prevalence was established by using e. histolytica stool antigen detection kits and a polymerase chain reaction (pcr) with genomic dna extracted from cultured trophozoites in all four-nuclei, amoeba-positive samples from a population living in macaparana in northeastern brazil. a ...200414993636
[the absence of invasive amebiasis in male homosexual aids patients in recife].the purpose of this paper was to determine if among male homosexual aids [correction of sida] patients, the entamoeba histolytica was able to product invasive illness. from the 77 investigated patients only one (1.3%) has cysts of e. histolytica in his stools. the electrophoresis of the only isolated strain has shown it was from zimodeme i, non pathogenic. the research of antiamebic antibodies was negative in the serum of the totality of patients. those results showed that even in immunocompromi ...19968768579
analysis of pathogenicity by restriction-endonuclease digestion of amplified genomic dna of entamoeba histolytica isolated in pernambuco, brazil.the pathogenicity of 47 strains of entamoeba histolytica isolated in pernambuco, brazil, was examined using the polymerase chain reaction (pcr) followed by restriction-endonuclease digestion. electrophoretic patterns of pcr products digested with hinfi revealed that all strains were nonpathogenic. the results were entirely in accord with phenotypic properties such as isoenzyme patterns and the failure to bind a pathogenic-isolate-specific monoclonal antibody. when the sensitivity of pcr was exam ...19921495923
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