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evaluation of newer diagnostic methods for the detection and differentiation of entamoeba histolytica in an endemic area.we evaluated the colorimetric polymerase chain reaction (pcr)-based method for the detection and differentiation of entamoeba spp. and compared the efficacy of e. histolytica-specific antigen detection in faeces with the detection of specific antibodies to e. histolytica-specific antigen in faeces, by enzyme-linked immunosorbent assay. faecal samples were obtained from patients attending hospital in chandigarh, india, from march 2001 to february 2002. the pcr-based colorimetric method was found ...200315259465
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