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survey of vibrio cholerae o1 and its survival over the winter in marine water of port of osaka.the survey of vibrio cholerae o1 in marine area was carried out in the port of osaka, japan in 1987-2001, and 51 v. cholerae o1 strains were isolated. all strains were identified to be of el tor biotype, ogawa serotype and classic ubon kappa-phage type, and were cholera toxin (ct)-negative and ct gene-negative. in order to clarify certain ecological aspects of v. cholerae o1 in the marine environment of the temperate zone, we performed molecular analysis of the isolated strains using pulsed-fiel ...200312948359
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