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cutaneous leishmaniasis in khyber pakhtunkhwa province of pakistan: clinical diversity and species-level diagnosis.this study primarily aimed to identify the causative species of cutaneous leishmaniasis (cl) in the khyber pakhtunkhwa province of pakistan and to distinguish any species-specific variation in clinical manifestation of cl. diagnostic performance of different techniques for identifying cl was assessed. isolates of leishmania spp. were detected by in vitro culture, polymerase chain reaction (pcr) on dna extracted from dried filter papers and microscopic examination of direct lesion smears from pat ...201627601518
phylogenetic position of leishmania isolates from khyber pakhtunkhwa province of pakistan.several species of the genus leishmania are causative agents of cutaneous leishmaniasis in pakistan. this study aimed to determine phylogenetic placement of leishmania species causing cutaneous leishmaniasis in khyber pakhtunkhwa province, pakistan (34 leishmania tropica, 3 leishmania infantum), in-relation to species from other geographical areas using gene sequences encoding cytochrome b (cytb) and internal transcribed spacer 2 (its2). based on cytochrome b sequence analysis, l. tropica strain ...201627233810
an outbreak of cutaneous leishmaniasis in an afghan refugee settlement in north-west pakistan.cutaneous leishmaniasis (cl) due to leishmania tropica appears to be an emerging disease in parts of north-east afghanistan and north-west pakistan. timargara, an afghan refugee camp of 17 years' standing, in the district of dir, north west frontier province of pakistan, experienced a major outbreak of cl in 1997 for the first time. as part of the investigation, each section of the camp was surveyed for cl. around 38% of the 9200 inhabitants bore active lesions and a further 13% had scars from e ...199910450434
cutaneous leishmaniasis: iso-enzyme characterisation of leishmania tropica.in order to identify and characterise the organisms responsible for cutaneous leishmaniasis, parasites were isolated from active lesions, grown in-vitro cultures and identified by iso-enzyme characterisation. thirteen isolates from different patients were typed as l. tropica. seven of these isolates were from afghan refugees encamped in the suburbs of islamabad, 3 were from patients in multan, 1 was from a patient from azad jammu and kashmir and 1 was from besham (swat, nwfp). the study confirms ...19979510629
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