| identification and differentiation of iranian leishmania species by pcr amplification of kdna. | we describe the specific identification of leishmania species in iran using pcr dna amplification of kdna. for this purpose, we designed a pair of primers--upstream 5' tcgcagaacgcccctacc 3' and downstream 5'-aggggttggtgtaaaataggc 3'--specific for conserved sequences of kdna of leishmania. using this primer, we identified 3 different amplified fragments from the kdna of the who reference leishmania species. two bands at 620 and 850 bp were identified for l. major (mrho/ir/64/nadim-1 strain) and o ... | 2001 | 11525354 |