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polymorphisms of cytochrome b gene in leishmania parasites and their relation to types of cutaneous leishmaniasis lesions in pakistan.the exact species and/or strains of leishmania parasites involved strongly influence the clinical and epidemiological features of leishmaniasis, and current knowledge of those influences and relationships is inadequate. we report that cytochrome b (cyt b) gene sequencing identified causal leishmania parasites of 69 cutaneous leishmaniasis cases in pakistan over a 3-year period. of 21 cases in highland areas (quetta city, balochistan province), 16 (76.2%) were identified as leishmania (l.) tropic ...200818271802
multilocus enzyme electrophoresis and cytochrome b gene sequencing-based identification of leishmania isolates from different foci of cutaneous leishmaniasis in pakistan.seventeen leishmania stocks isolated from cutaneous lesions of pakistani patients were studied by multilocus enzyme electrophoresis and by polymerase chain reaction amplification and sequencing of the cytochrome b (cyt b) gene. eleven stocks that expressed nine zymodemes were assigned to l. (leishmania) major. all of them were isolated from patients in the lowlands of larkana district and sibi city in sindh and balochistan provinces, respectively. the remaining six, distributed in two zymodemes ...200616896129
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